百色市山羊布鲁氏菌病及衣原体病血清流行病学调查报告

为掌握百色市山羊布鲁氏菌病及衣原体病感染状况。在2013年对百色市辖12个县区的养羊场进行调查并采集血清进行布病及衣原体检测。调查发现,1732个羊场中有159个发生流产、死胎的现象。431个存栏100只以上的羊场中,有87个发生过流产、死胎现象。对76个羊场采集924份血清样品使用虎红平板凝集试验及试管凝集试验进行检测,未检出布鲁氏菌阳性血清。送检520份血清,检出衣原体阳性血清52份,阳性率10%;其中检测种母羊血清139份,检出衣原体阳性血清14份,阳性率10.07%;检测种公羊血清72份,检出衣原体阳性血清9份,阳性率12.50%。经分析,百色市山羊的布病防控达到广西稳定控制标准要求;羊衣原体病在本场乃至整个百色地区有蔓延流行的风险。     

靖边县2006—2010年畜间布鲁氏杆菌病血检分析

通过对靖边县2006年至2010年畜间布鲁氏杆菌病血检情况的分析,得出靖边县从2006年发生畜间布鲁氏杆菌病后,经过5年的艰苦防治,取得了一定成绩,畜间布鲁氏杆菌病阳性率明显下降.并结合畜间布鲁氏杆菌病发病情况,提出今后布鲁氏杆菌病综合防控的建议.     

First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors,explaining differences between geographical origins

BackgroundBrucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here we report the first genome sequencing of a B. canis strain in Chile, and the differences in virulence genes with other B. canis strains.ResultsGenome assembly produced a total length of 3,289,216 bp, N50 of 95,163 and GC% of 57.27, organized in 54 contigs in chromosome I, and 21 contigs in chromosome II. The genome annotation identified a total of 1981 CDS, 3 rRNA and 36 tRNA in chromosome I, and 1113 CDS and 10 tRNA in chromosome II. There is little variation between the different strains and the SCL isolate. Phylogenetic analysis showed that the Chilean SCL strain is closely related to B. canis and B. suis strains. Small differences were found when compared to the Serbian isolate, but all strains shared the same recent common ancestor. Finally, changes in the sequence of some virulence factors showed that the SCL strain is similar to other South American B. canis strains.ConclusionsThis work sequenced and characterized the complete genome of B. canis strain SCL, evidencing the complete presence of all the genes of the virB operon, and minor changes in outer membrane proteins and in the urease operon. Our data suggest that B. canis was introduced from North America and then spread throughout the South American continent.How to cite: Borie C, Bravo C, Dettleff P, et al. First genome sequence of Chilean Brucella canis SCL strain provides insights on the epidemiology and virulence factors, explaining differences between geographical origins Electron J Biotechnol 2021;49. https://dx.doi.org/10.1016/j.ejbt.2020.10.002.