Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk

Specific immunoglobulin （IgY） from egg yolk against Aeromonas hydrophila was produced by immunization of White Leghorn hens with formalin-killed whole cells of A. hydrophila. ELISA test using A. hydrophila as the coating antigen revealed that the specific antibody titer started to increase in the egg yolk at the 13th day post-immunization （P/N=2.18）, reached the peak at the 56th day （P/N= 13.82）, and remained at high level until day 133 （P/N=7.03）. The antibody was purified by saturated ammonium sulphate with a recovery rate of 63.5%. The specific IgY inhibited the growth ofA. hydrophila at a concentration of 1.0 mg/ml during the 18 h incubation. Pre-treatment of polyploid gibel carps Carassius auratus Gibelio with specific IgY had a protection rate of 60% （6/10） against challenge with A. hydrophila, while none of the fishes in the control groups receiving sterile phosphate buffered saline （PBS） or non-specific IgY survived the challenge. Treatment of fishes with the specific IgY 4 h after the challenge also had lower mortality （70%, 7/10）, a 30% reduction against the control PBS or non-specific IgY groups （10/10）. These results indicate that specific IgY antibodies could be obtained easily from hens immunized with an inactivated A. hydrophila and could provide a novel alternative approach to control of diseases in fishes caused by this organism.     

长江黄州段幼鲤体内寄主蠕虫的调查及鱼类资源的合理利用

１９９９年１１月上旬,对长江黄州江段的鲤鱼进行了相对渔获量、种群结构和体内寄生蠕虫的调查。统计结果表明,鲤鱼只占总渔获量的７％,且９４％为当年的小幼体。鲤鱼的鳃上及消化道内只有三种蠕虫寄生。宿主感染这三种寄生蠕虫的感染率和感染强度均很小。据此提出保护、合理利用这一鱼类资源的几点建议。     

《饮马长城窟行》新解——兼释"双鲤鱼"

着重对诗中的“双鲤鱼”意象展开讨论，用实证的方法探寻鱼形书函的起源，归总了“双鲤鱼”在《饮》诗中的多重含义：不仅实指鱼形书函，还有诚信之意，并且特别蕴含了“男女好合”的意思，是爱情的象征。在此基础上，得出对《饮》诗新的理解。