被子植物精细胞分离和特性研究进展

精细胞分离是研究雄性生殖发育的前提之一,近半个世纪以来,人们运用不断更新的生物实验技术,对被子植物精细胞开展了分离及细胞分子特征的研究。分析被子植物精细胞分离方法,总结精细胞二型性与倾向性、细胞融合方面的研究成果,并对其研究意义和前景进行讨论。     

柴油机燃油喷射系统的仿真计算及分析

较全面地分析了柴油机燃油喷射系统仿真计算的影响因素，给出了仿真计算的基本数学模型。研究并分析了传统和电控喷油系统仿真计算的特点，分析了现有计算模型的现状及部分缺陷，并就其发展提出了一些建议。     

基于Dialog联机检索的行业专利分析研究

本文基于Dialog联机检索，以德温特世界专利索引数据库和专利引文索引数据库为数据源，对质子交换膜（PEM）燃料电池行业进行了专利分析研究。作者用实例说明了利用各种Dialog高级检索指令实现行业专利分析的技巧。通过分析研究，发现了PEM燃料电池行业的领先企业、技术领军人物、产业发展前景、全球技术／市场布局、关键技术、研发热点等。研究表明，Dialog？联机检索在行业专利分析中是一种有效的工具。     

新型化学电源--燃料电池

燃料电池是一种等温并直接将储存在燃料和氧化剂中的化学能高效、环境友好地转化为电能的新型发电装置．燃料电池不受热机效率的限制，能量转换效率高，无污染、无噪音，具有满足环境保护的要求．预计燃料电池系统将在洁净煤燃料电池电站、电动汽车、移动电源、不间断电源、潜艇及空间电源等方面有着广泛的应用前景和巨大的潜在市场．本文介绍了燃料电池的的优点、主要种类、工作原理、国内外发展概况以及燃料电池的前景展望。     

液化石油气发动机燃料供给系统的设计方案与性能比较研究

液化石油气(LPG)发动机燃料供给系统已经历了三个发展阶段,国外已开发出第三代产品。将三代LPG发动机燃料供给系统的设计方案作了简要介绍,并对其性能作了比较分析,从中可了解国外LPG发动机燃料供给系统的发展历程以及各阶段的技术关键,供国内同行参考。     

In vitro study on ribozyme against hepatitis C virus

Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.     

水稻胚乳细胞发育与结实特性

文章对水稻胚乳细胞发育过程、淀粉体和蛋白体结构特征与结实特性、养分输入胚乳细胞的途径、ATPase活性分布与水稻充实的关系、水稻强弱势粒胚乳细胞发育与其结实特性进行分析.提出进一步研究胚乳细胞发育及其超微结构与籽粒生理活性的关系,可从深入开展亚显微水平下强弱势粒背部维管束结构、ATPase的活性分布、差异表达基因对结实特性的影响等方面展开研究.     

基于ANSYS的卡车燃油箱结构强度分析

卡车燃油箱是燃油供给系统的核心部件,其设计研发主要依靠工程师的经验来完成,产品更新速度慢,成本高.结合现代化设计方法利用三维软件UG设计出卡车油箱的三维结构模型,然后利用有限元方法对卡车燃油箱结构进行应力应变分析,求出卡车油箱的最大应力值σmax为319.237 MPa,最大应变值εmax为13 mm,为卡车油箱的设计修改提供理论参考依据.大大降低了卡车油箱的设计成本,提高了产品研发效率.     

Mapping the knowledge structure exhibited by a cohort of students based on their understanding of how a galvanic cell produces energy

This study evaluates the use of an open‐ended question to determine students' knowledge structure on the topic of galvanic cells. The open‐ended question was developed and administered to 163 Grade 10 students who had earlier completed a course on electrochemistry. Students' responses were marked as well as coded on the basis of the fields identified from their responses. This was then evaluated statistically to determine the collective knowledge structure of the sample of students. The knowledge structure thus mapped contains both canonical concepts and alternative conceptions (ACs). An important finding emerging from this study is that instructors need to focus student's attention on the dynamic processes involving electrons and ions during the operation of galvanic cells. In order for students to fully understand how a galvanic cell operates, they need to see the whole picture. There are three critical components that lead to students' understanding of how an oxidation–reduction reaction can generate energy and how a circuit is complete: transfer of electrons during oxidation–reduction half‐reactions, flow of electrons within metals, and migration of ions in solution. Also, we found that it is possible for students to use correct chemistry concepts in an incorrect way by establishing linkages among these in an inappropriate manner. We reiterate that apart from evaluating students' ACs, it is also important to evaluate the links between the concepts and conceptions present in students' knowledge structure so that teaching can be made more effective.     

Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart

Objective: To investigate the directed transplantation of allograftic bone marrow-derived mesenchymal stem cells (MSCs) in myocardial infarcted (MI) model rabbits. Materials and Methods: Rabbits were divided into 3 groups, heart infarcted model with MSCs transplanted treatment (MSCs group, n=12), heart infarcted model with PBS injection (control group, n=20), sham operation with PBS injection (sham group, n=l 7). MSCs labelled by BrdUrd were injected into the MI area of the MSCs group. The same volume of PBS was injected into the MI area of the control group and sham group. The mortality, LVIDd, LVIDs and LVEF Of the two groups were compared 4 weeks later. Tropomyosin inhibitory component (Tn I) and BrdUrd immunohistochemistry identified the engrafted cells 4 weeks after transplantation. Result: The mortality of the MSCs group was 16.7% (2/12), and remarkably lower than the control group's mortality [35% (7/20) (P＜0.05)].Among the animals that survived for 4 weeks, the LVIDd and LVIDs of the MSCs group after operation were 1.17±0.21 cm and 0.74±0.13 cm, and remarkably lower than those of the model group, which were 1.64±0.14 cm and 1.19±0.12 cm (P＜0.05); the LVEF of the MSCs group after operation was 63±6%, and remarkably higher than that of the model group,which was 53±6% (P＜0.05). Among the 10 cases of animals that survived for 4 weeks in the MSCs group, in 8 cases (80%),the transplanted cells survived in the non MI, MI region and its periphery, and even farther away; part of them differentiated into cardiomyocytes; in 7 cases (70%), the transplanted cells participated in the formation of blood vessel tissue in the MI region. Conclusion: Transplanted allograftic MSCs can survive and differentiate into cardiomyocytes, form the blood vessels in the MI region. MSCs transplantation could improve the heart function after MI.