CHOP及GRP78在复发性多形性胶质母细胞瘤患者表达显著增加

目的:研究CHOP及GRP78与复发性GBM的相关性。方法:采用Western blot检测CHOP及GRP78在初发及复发GBM组织中的表达;Real-time PCR检测GRP78在初发以及复发GBM组织中的表达。结果:CHOP及GRP78在初发GBM中表达上调,而在经过原发性肿瘤切除后接受放疗和替莫唑胺化疗的复发性GBM中增加尤为显著。结论:CHOP及GRP78在GBM发病机制中起重要作用,该分子有望成为GMB潜在的诊断及预后评估标志物。     

大鼠神经胶质瘤和嗜铬细胞瘤中P2X受体表达特征的研究

目的：采用实时荧光定量聚合酶链式反应（polymerase chain reaction,PCR）技术,研究P2X受体在大鼠神经胶质瘤和嗜铬细胞瘤及原代培养皮层神经元和星形胶质细胞上的表达差异。方法：取新生1-2 d SD大鼠大脑皮层,分离纯化神经元和星形胶质细胞,并采用实时荧光定量PCR技术,比较P2X受体在大鼠胶质瘤C6细胞、大鼠肾上腺嗜铬细胞瘤PC-12细胞、星形胶质细胞和皮层神经元上的表达差异。结果：C6细胞P2X2、P2X3和P2X5表达水平显著高于星形胶质细胞,P2X4、P2X6和P2X7表达水平显著低于星形胶质细胞,PC-12细胞P2X1、P2X2、P2X3和P2X6表达水平显著高于皮层神经元,P2X5和P2X7表达水平则显著低于皮层神经元。此外,还发现P2X2、P2X5和P2X6在C6和PC-12细胞上的表达水平存在显著差异。结论：大鼠胶质瘤和嗜铬细胞瘤细胞表达多种P2X受体,且与原代培养细胞存在表达差异,提示核苷酸介导的信号传递系统可能作为潜在的肿瘤治疗靶点。     

Prediction of malignancy selective neural networks degree in brain glioma using ensemble

A clustering algorithm based selective neural networks ensemble （CLUSEN） is proposed to predict the degree of malignancy in brain glioma. Since the degree prediction of malignancy is critical before brain surgery, many learning methods are used like rule induction algorithm, single neural networks, support vector machines, etc. Ensemble learning methods can improve the generalization of single learning machine, and are becoming popular in the machine learning and medical data processing communities. The procedure of CLUSEN can efficiently remove redundancy learning individuals and help improve the diversity of ensemble methods. CLUSEN is used to predict the degree of malignancy in brain glioma. Experimental results on a set of brain glioma data show that, compared to support vector machines, rule induction and single neural networks, the classification accuracy of CLUSEN is higher.     

基于聚类算法的选择性神经网络集成在大脑胶质瘤诊断中的应用

1 Introduction The degree of malignancy in brain glioma[1]domi-nates the way of treatment .In case of gradeⅠorⅡaccording to Kernohan,the success rate of operationis satisfactory. Otherwise ,for gradeⅢor IV,the sur-gical risk is high ,and poor life qual…     

颅内肿瘤114例临床病理分析

本文回顾性分析了我院114例颅内肿瘤的类型、年龄分布、性别比较、发病部位。并就胶质瘤的病理组织学分类,颅内分布及肿瘤类型与手术预后关系进行了讨论.