丝素改性胶原膜的肝素化

以1-(3-二甲氨基丙基)-3-乙基碳二亚胺(简称EDCI)为缩合剂对丝素改性胶原膜进行了肝素固定化,用X光电子能谱分析了材料的表面性质. 测定了固定化肝素的量并研究了固定化肝素的稳定性. 结果表明固定化肝素量为2.34μg/cm-2,体外释放试验表明肝素量虽有下降但有较好的稳定性.     

高通量药物筛选中靶分子固定化数学物理模型的建立

通过对高能量药物筛选基本过程的分析 ,探索了靶分子固定化可能对筛选质量构成的影响 .从分子相互作用看 ,实际的过程远较理想的情况复杂 .分子间存在的静电力、疏水作用力及氢键力能对固定化的机理构成较大影响 ,甚至可能诱发多层吸附的固定化模式 .基于固定化过程中可能存在的靶分子与靶分子、靶分子与微球、体相与表面相之间的相互作用 ,构筑了相应的数学物理模型 ,为实际的筛选工作提供了可参考的理论依据 .     

辣根过氧化物酶酶学特性及应用进展

辣根过氧化物酶（HRP）是一种以亚铁血红素为氧化还原中心的过氧化物酶,从植物辣根的根部提取,目前被广泛地应用在污水处理、食品工业、有机合成和分析检测等领域。本文综述了辣根过氧化物酶的结构、固定化及辣根过氧化物酶在各个领域的应用,并对其工业应用前景及未来研究方向进行了探讨。     

Design and synthesis of chiral Ti-1,1-bi-2-naphthol coordination polymers for heterogeneous catalytic asymmetric oxidation of sulfides

Polymer-immobilized catalysis has many advantages such as easy recovery and reuse of catalyst. We prepared three novel chiral 1,1＇-bi-2-naphthol-Ti coordination polymers with properly designed ligands and Ti（O^ipr）4 under mild conditions. The prepared polymers exhibited good activity and excellent enantioselectivity （over 99%ee） in catalyzing the asymmetric oxidation of sulfides. The bridge linker in the polymer and the reaction solvent noticeably affected the enantioseleetivity. The chiral coordination polymer was very stable and easy to separate from catalyzed reaction systems, with no significant loss of activity or enantioselectivity after reuse for at least ten times. These findings suggest a promising type of catalysts for synthesizing the widely used sulfoxides by asymmetrically oxidizing sulfides.     

Preparation of Glutaraldehyde Cross-Linked Chitosan Beads Under Microwave Irradiation and Properties of Urease Immobilized onto the Beads

The glutaraldehyde cross-linked chitosan beads were prepared under microwave irradiation and urease was immobilized onto the beads. The activity and the yield of enzyme activity of the immobilized urease were 10.83 U/g carrier and 47.7%, respectively. The optimum conditions of immobilization were 1% of glutaraldehyde volume fraction, 10 mg/g of urease/beads weight ratio, 24 h of the processing time and pH 6.5 of the reaction medium for immobilization. The properties of the immobilized urease were investigated and compared with those of the free enzyme. The optimum pH values were 6.5 and 7.0 for the immobilized and free urease, respectively. The optimum temperature was 60℃ for the free urease, while it shifted to 65 ℃ for the immobilized enzyme. The Michaelis constant Kr, was 9.1 mmol/L for the immobilized and 12.5 mmol/L for the free urease. The immobilized urease retained 40% of its initial enzyme activity even after 10 repeated uses. The immobilized urease stored at 4 ℃ retained 46% of its initial activity even after 35 d.     

TiO2光催化剂固定化载体及固化方法

介绍了TiO2光催化剂载体的选拔依据和作用，阐述了纳米TiO2的固化方法。     

黄孢原毛平革菌胞外过氧化物酶系的固定化及性质研究

通过共价结合法将木素过氧化物酶(Lip)与锰过氧化物酶(Mnp)固定于聚氨酯泡沫上.固定化酶的Lip与Mnp活力分别为1.45 U/g(泡沫干重)和0.49 U/g(泡沫干重),酶活回收率分别达到34.79%和27.78%.固定化酶Lip和Mnp热稳定性提高,在温度达到50℃时酶活损失均少于20%.与游离酶相比,固定化酶稳定的pH范围前后都扩大了0.5个单位.固定化酶重复利用性好,连续使用6次,酶活力损失少于3%.     

IMMOBILIZED ASPERGILLUS ORYZAE CELLS AND THEIR ACTIVITIES

采用戊二醛－明胶－乙二胺将氨基酰化酶交联固定在米曲霉细胞上，应用正交实验设计优选出相应的固定化条件，制备出具有较高反应活性和稳定性，适用于拆分ＤＬ－氨基酸的固定化细胞，并就ｐＨ、温度、离子浓度、底物浓度等因素对固定化细胞反应活性的影响进行了实验研究．分别在固定化细胞柱（ＩＣＣ）和新型固定化细胞－膜耦联反应器（ＣＩ－ＣＢＭＲ）中进行了连续光学拆分Ｎ－乙酰－ＤＬ－丙氨酸的实验研究，结果表明该固定化细胞具有良好的工业应用前景．     

高频正弦波振动对制动大鼠比目鱼肌MHC表达的影响

目的探讨高频正弦波振动(HFV)对制动大鼠比目鱼肌(SOL)梭内、外肌纤维肌球蛋白重链(MHC)表达的影响。方法采用大鼠后肢制动作为废用性肌萎缩动物模型,对振动组大鼠施以HFV,用免疫组化染色法检测大鼠SOL梭内、外肌纤维MHC表达。结果①制动14 d后,大鼠SOL梭外肌Ⅰ型肌纤维的构成比明显减少,Ⅱ型肌纤维的构成比明显增加(P<0.05),SOL梭内肌纤维快缩型MHC的表达有所增强;②制动加HFV大鼠与制动组相比,SOL梭外肌Ⅰ型肌纤维的构成比明显上升,Ⅱ型肌纤维的构成比明显下降(P<0.05),SOL梭内肌纤维快缩型MHC的表达没有明显变化。结论 HFV对制动大鼠SOL梭外肌Ⅰ型肌纤维向Ⅱ型肌纤维的转化和梭内肌MHC表达增强的趋势有明显的对抗作用。     

大鼠右后肢固定及康复期参加体力活动对跟骨的影响

用石膏固定42只大白鼠的右后肢4周,去固定后第一、二、四周参加慢跑训练(左后肢供自身对照).用软X线及CT底片拍照,经光学显微镜放大35倍,计算跟骨骨小梁数目;直接测量跟骨各径线、体积;用X线骨密度仪测定跟骨结节的骨密度.结果表明,固定4周后,跟骨宽径及体积变大、变形;骨小梁数目减少.康复慢跑训练,右跟骨骨膜反应.足、踝损伤经石膏固定的运动员,提踵能力下降,不敢持重,不敢完成跳跃落地动作,其基本病理变化之一是骨质疏松.