Acrosin activity in spermatozoa of infertile Nigerian males

The objective of this study was to evaluate acrosin activity in spermatozoa of infertile Nigerian men and to compare with those of the fertile men. The acrosin activity was evaluated using the Acroscreen reagent kit. The acrosin activity of the fertile men was 40.7±5.2 mIU/10⁶ sperm (range 30.1–51.3) and those of the infertile men was 22.4±8.33 mIU/10⁶ sperm (range 5.7–39.1). The difference in the mean was statistically significant (P<0.001). There was a subpopulation of the infertile men who had their acrosin activity within normal range of the fertile men, 32.9±1.57 mIU/10⁶ sperm. But the difference in the mean was statistically significant (P<0.001). Acrosin activity decreased with increased morphological changes in the spermatozoa. It is concluded that acrosin activity in the infertile Nigerian men is significantly lower than that in the fertile men. Acrosin activity may also be affected by morphological changes in the spermatozoa.     

Laboratory medicine in pandemic of COVID-19

After the outbreak in China in the year 2019, severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) quickly spread around the world causing a protracted pandemic. Approximately one-third of infections appear to be asymptomatic. Symptomatic disease is characterized primarily by symptoms of respiratory tract infection of varying severity. But Coronavirus Disease 2019 (COVID-19) is much more than an acute respiratory disease because SARS-CoV-2 affects many organs inducing a vast number of symptoms such as cardiovascular, neurological, gastrointestinal, dermatological, with numerous complications. Short and long-term effects of infection, severe ones, and especially mild forms of the disease which affect a huge number of patients need to be further investigated. Laboratory medicine has a crucial role in early diagnosis of the disease, recognition of the patients who need hospital care, and close monitoring of hospitalized patients to timely identify associated clinical complications as well as follow-up of patients with long-term COVID-19.     

ICSI治疗前无精子症患者Y染色体微缺失的筛查分析

目的　研究ICSI治疗前无精子症患者Y染色体微缺失发生率及微缺失类型,探讨精子生成障碍与Y染色体微缺失的关系.方法　应用PCR技术,对63例ICSI前行诊断性睾丸活检的无精子症患者进行Y染色体15个序列标记位点的微缺失检测.结果　63例无精子症患者中发现12例有Y染色体微缺失,缺失率为19.1%.其中10例患者的缺失发生在AZFc/DAZ区域,缺失率为15.8%;2例患者发生AZFb、AZFc双重缺失,缺失率为3.2%,AZFa无缺失病例.结论　Y染色体微缺失是引起男性无精子症的重要原因之一.采用PCR技术进行微缺失检测是一种非常有效的方法.对无精子症患者进行ICSI治疗前,有必要进行Y染色体的微缺失筛查.     

不孕妇女BV与AsAb的关系探讨

目的研究不孕妇女细菌性阴道病（BV）与抗精子抗体（AsAb）的关系。方法对371例不孕妇女进行BV检测,同时采用间接酶联免疫吸附试验（ELISA）测定血清、宫颈粘液中的AsAb水平。结果血清AsAb阳性率BV组32．7％,非BV组15．2％;宫颈粘液AsAb阳性率BV组37．6％,非BV组15．9％;经X2检验,两组间均有显著性差异（p〈0．01）。结论细菌性阴道病与抗精子抗体的产生有相关性,与女}生免疫性不孕的形成和发展有一定的关系。     

具有阶段结构的不育控制害鼠模型

着重讨论了一类具有阶段结构的非自传播的不育害鼠模型,分析了各个平衡点的局部渐近稳定性,并给出各平衡点全局渐近稳定的数值模拟,讨论了不育比例β对于种群规模的影响。     

不育夫妇病因诊断程序优化设计研究

目的:为不孕症的病因诊断,设计容易管理且系统全面的应用程序,并将相关资料进行总结与统一管理,便于开展进一步的应用研究使不育夫妇能尽快找到病因以便指导随后的治疗,避免延误治疗。方法:查找并总结不孕症病因诊断的各种相关资料,结合本院研究所的经验与附属医院的技术及设备,建立切实可行的不育夫妇病因查找方法和程序、相关资料的电子档案。结果:确立不孕症病因诊断程序并附加各种病因诊断定义标准,建立不育夫妇病因诊断结果的电子档案管理系统。结论:本课题成果应用于临床可以指导不孕症病因诊断过程、缩短不育夫妇的诊治周期。     

宫腔镜手术治疗继发不孕36例临床分析

目的:探讨宫腔镜手术对继发不孕的诊断及治疗价值.方法:对2003年1月至2004年7月在我院治疗的36例继发不孕患者,在宫腔镜下实施1-2种手术,包括宫腔镜下胎骨碎片取出、宫腔粘连分离、疏通输卵管.结果:36例患者手术成功34人,成功率为94.4%,一年内妊娠20人,妊娠率为55.6%.结论:宫腔镜手术只需局部封闭麻醉,通过手术可发现继发不孕的原因所在并及时地处理祛除病因,提高了治愈率.     

大学教师“不育症”应诊

培育人才是大学诞生的第一动力,也是大学教师的职业本色。然而,当前却有一些大学教师＂只教书,不育人＂,得了＂不育症＂。医治大学教师＂不育症＂,不能完全按传统做法＂问题——原因——对策＂入手,而应该变革当前高校教师的综合评价模式,尽快理顺大学教师的职责和任务。     

Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction

The excessive generation of reactive oxygen species (ROS) by abnormal spermatozoa and contaminating leukocytes has been defined as one of the few etiologies for male infertility. Administration of antioxidants in patients with ‘male factor’ infertility has begun to attract considerable interest. The main difficulty of such an approach is our incomplete understanding of the role of free radicals in normal and abnormal sperm function leading to male infertility. Mammalian spermatozoa membranes are very sensitive to free radical induced damage mediated by lipid peroxidation, as they are rich in polyunsaturated fatty acids. Limited endogenous mechanisms exist to reverse these damages. ROS attacks the fluidity of the sperm plasma membrane and the integrity of DNA in the sperm nucleus. ROS induced DNA damage accelerate the germ cell apoptosis. Unfortunately spermatozoa are unable to repair the damage induced by excessive ROS as they lack the cytoplasmic enzymes required to accomplish the repair. Assessment of such oxidative stress status (OSS) may help in the medical treatment. Treatment strategies must be directed toward lowering of ROS levels to keep only a small amount necessary to maintain normal cell function.     

免疫不育控制下的种群动态分析

建立了以病毒为媒介的不育种群动力学模型,从理论上探讨各平衡点存在性,并通过构造Liapunov函数,Dulac函数等证明了各平衡点的全局稳定性。