心脏Nmnat过表达联合耐力运动对果蝇高脂膳食诱发脂毒性心肌症的影响

利用UAS/hand-gal4系统构建果蝇心脏Nmnat基因特异性过表达,研究心脏Nmnat基因过表达联合耐力运动对高脂膳食诱发脂毒性心肌症的影响,并分析其分子机制。方法:雄性w^1118和NmnatUAS系果蝇分别与雌性hand-Gal4杂交,收集F1代心脏Nmnat正常表达(hand>w^1118)和过表达(hand>Nmnat)处女蝇,饲养至15 d后开始进行耐力运动(E)和高脂膳食(HFD),并分为hand>w^1118、hand>w^1118+E、hand>w^1118+HFD、hand>w^1118+HFD+E、hand>Nmnat、hand>Nmnat+E、hand>Nmnat+HFD、hand>Nmnat+HFD+E共8组,每组410只。ELISA检测心脏TAG、NAD+、SIR2蛋白去乙酰化、SOD活力和MDA水平,qRT-PCR检测心脏相关基因mRNA表达,M-mode检测心脏功能情况。结果:hand>w^1118+HFD+E果蝇心脏的Nmnat、dFoxo、bmm表达、NAD+水平、SIR2去乙酰化、SOD活力、缩短分数高于hand>w^1118+HFD(P<0.05或P<0.01),且d FAS和Col4al表达、TAG和MDA水平、心率和纤维性振颤低于hand>w^1118+HFD(P<0.05或P<0.01);hand>Nmnat果蝇心脏各指标与hand>Nmnat+HFD相比较未见显著差异(P>0.05);hand>Nmnat+HFD+E果蝇心脏的Nmnat、dFoxo、bmm表达、心脏NAD+水平、SIR2去乙酰化、SOD活力、心脏缩短分数高于hand>Nmnat+HFD(P<0.05或P<0.01),且dFAS和Col4al表达\TAG和MDA水平、心率和纤维性振颤低于hand>Nmnat+HFD(P<0.05或P<0.01)。结论:心脏Nmnat过表达和耐力运动均能抵抗果蝇高脂膳食诱发的心脏脂质过度堆积、氧化损伤、减弱心脏收缩能力和增加纤维性振颤,其分子机制与心脏Nmnat/NAD+/SIR2/dFoxo通路活性增强有关。心脏Nmnat基因过表达联合耐力运动能更好地降低脂毒性心肌症的发生概率,其机制与二者联合对心脏Nmnat/NAD+/SIR2/dFoxo通路活性上调的叠加作用有关。     

专利制度与经济增长:理论与现实--对中国专利制度运行绩效的评估

本文从激励创新和促进经济增长两个方面考证专利制度的绩效，在理论论证的基础上，就中国专利制度运行中的相关数据进行了全面的实证分析。结论表明，专利制度的绩效与制度的完善程度是关联的，现阶段对我国专利制度的完善应重视对原创性技术的倾斜及对专利技术商业化环境的完善。     

现代资本结构理论探析

阐述了现代资本结构理论的研究成果，评析了主流理论的主要内容和发展过程，同时对资本结构理论的新发展-资本结构决定因素学派作了概述和分析，指出该学派侧重实证角度研究影响企业资本结构的因素，为企业融资决策提供新思路。     

论福建省金融集聚对区域经济增长的影响

论述随着区域经济的快速发展,金融资源在空间地域集中已经成为普遍的经济现象。在金融成为现代经济发展核心的当代,金融集聚对经济增长有着至关重要的影响。运用区位商和格兰杰因果检验等方法,深入分析了福建省1990—2012年金融集聚与经济增长的内在联系,结果发现金融集聚是经济增长的格兰杰原因。提出为了促进福建区域经济增长,应采用合适的金融集聚模式,改进完善金融政策法制环境,搞好金融资源的合理分配与合作,吸引高端金融人才等建议。     

Surgical outcomes and strategy of hypertrophic obstructive cardiomyopathy

Objective： To evaluate the surgical clinical results of hypertrophic obstructive cardiomyopathy. Methods： We retrospectively collected data on 24 patients who underwent surgical management in the past ten years in two hospitals in China and Madras Medical Mission in India. Myomectomy was carried out on all patients. Among them 3 patients underwent mitral valve replacement; 2 patients underwent mitral valve repair （anterior mitral leaflet plication）; 2 patients underwent aortic valve replacement; 1 patient underwent aortic valve repair; 2 patients underwent aortic root replacement; 1 patient underwent Bentall＇s procedure and 1 patient underwent coronary artery bypass grafting because of a breached muscle bridge, Results： One patient died of post-operative heart failure. The mean follow-up time was 4.3 years, There was significant improvement in the symptomatic status. Sixteen patients were asymptomatic with good effort tolerance and only four patients had New York heart association （NYHA） Classes Ⅰ-Ⅱ due to associated valvular lesions, Conclusion： Our experience proved that symptomatic hypertrophic obstructive cardiomyopathy or non-symptomatic hypertrophic obstructive cardiomyopathy with combined heart disease is indication for surgery as surgical intervention could get better clinical results in this kind of patients compared with other non-surgical method because it beneficially reduces the systolic anterior motion （SAM） of the mitral valve leaflet, which could not be avoided by other non-surgical treatment.     

Serum lipids in dilated cardiomyopathy

Serum total cholesterol and triglyceride levels were estimated in 45 dilated cardiomyopathy (DCM) patients and were compared with 31 healthy controls. The total serum cholesterol (215±50 mg/dl) and serum triglyceride (162±76 mg/dl) levels in DCM patients did not show any significant changes as compared to normal controls. The cholesterol and triglyceride and the individual lipoprotein fractions, viz HDL, VLDL, LDL and chylomicrons were analysed in 10 patients. The values did not differ significantly when compared to those of controls. These findings suggest that serum lipids may not play a role in the antietiopathogenesis of DCM.     

Theoretical and Experimental Study on Ground Coupled Heat Pump with Vertical Spiral Coil

Groundcoupledheatpump (GCHP)isalsoclassi callycalledthegroundsourceheatpump ,ortheearthcoupledheat pump ,orthegeothermalheat pump ,thoughtheydonotreallyutilizegeothermalenergyintheclassicalsense .Insteadofhavingitsoutsidecoilsexchangeheatwithair ,itexchangesheatwiththeearthviagroundloopheatexchanger .Itdoesnotutilizegroundwaternordoesitneedreinjectionwellsoranyprotectionagainsterosion ,sotheycanavoidextensiveuseofgroundwaterandtheearthsubsidence .Withnopollutionofwasteheat ,smoke ,andwater,i…     

黟县在攀缘现代教育技术制高点

介绍了安徽省黟县大力普及信息技术教育，实施“校校通”工程的过程和经验。     

解读Quirk看“虚拟”--兼答刘礼进先生

从Quirk等有关虚拟语式重要性方面的观点的变化着手,探讨了“两型说”及其起源、Quirk等与章振邦等在类型划分上的异同、“两型说”的缺陷及刘礼进(2001)对Quirk等的误读,并指出,作为虚拟语式重要特征的“句法从属性”是客观存在,无法否认的。在解答刘礼进(2001)有关问题时,作者对“后时虚拟式”的最早观点作了部分修正,提出：“命令虚拟式”体现“语境后时”,“程式虚拟式”体现“意念后时”。     

说唱音乐浅论

在我国民族民间音乐中，说唱音乐有其特有的，不可替代的地位和作用，这表现在两个方面。第一，说唱音乐的历史悠久，它同社会的发展，人民的生活息息相关。第二，说唱音乐是我国民族民间音乐发展中的里程碑，这表现为两点：一是说唱音乐继承和发扬了民歌的精髓，孕育和促进了戏曲的产生，二是说唱音乐的发展也促进了民间器乐的发展，丰富了民间器乐的表演形式和内容。