结晶紫催化动力学光度法测定微量草酸

在稀磷酸介质中,微量草酸对KBrO3氧化结晶紫的褪色反应有显著的活化作用,据此建立了测定微量草酸的新方法。方法的线性范围是O.5-5.0μg/mL,检出限为O.2μg/mL.方法简便,快速,灵敏度高,用于茶叶中草酸含量的测定,结果满意。     

甲基橙褪色光度法测定加碘盐中微量碘

基于硫酸介质中,在溴化钾催化作用下,碘酸根氧化甲基橙褪色作用,据此建立了测定碘的动力学光度法.结果表明,最大吸收波长为507 nm时,碘酸钾浓度在0~1.6 mg/L内呈线性关系,表观摩尔吸收系数为9.17×104L.mol-1cm-1.方法用于加碘盐中碘(V)的测定,结果令人满意.     

A rapid and accurate 3-（4,5-dimethyl thiazol-2-yl）-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125～32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7～8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24～28 h for ADA. The MCA provides an accurate and rapid method for quantifi-cation of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

重组猪囊尾蚴cC1蛋白用于囊尾蚴病人血清抗体的检测

诱导、表达含有猪囊尾蚴cC1蛋白基因的原核表达载体pET-28a质粒(pET28a-cC1),纯化、鉴定融合蛋白,以此作为抗原包被反应板,对囊尾蚴病人等血清进行ELISA检测。检测56份囊尾蚴病患者血清,抗体阳性者53份,阳性率为94.7％;检测30份血吸虫病患者血清,交叉反应率为3.33％(1/30);检测30份正常...     

系统性红斑狼疮患者血清铁蛋白水平检测

用酶联免疫吸附试验测定２８例系统性红斑狼疮（ＳＬＥ）患者血清铁蛋白水平，结果表明，与正常对照比较ＳＬＥ患者血清铁蛋白水平增高，但治疗前后血清铁蛋白改变无显著性，与疾病活动指数无显著相关性．这些结果提示ＳＬＥ患者存在有异常铁代谢，本文对此进行了讨论．     

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp简

A novel monoclonal antibody (MAb) against oxytetracycline (OTC) was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay (ELISA)-based detection system. An OTC-bovine serum albumin (BSA) conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2–4F, 7–3G, and 11–11A. An indirect competitive ELISA (icELISA) was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration (IC50) and percentage of cross-reactivity, respectively. MAb 2–4F exhibited the highest sensitivity, with an IC₅₀ of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no cross-reactivity to other unrelated antibiotics. When MAb 2–4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%–118% for an intra-assay and 96%–113% for an inter-assay. The coefficients of variation of the assays were 3.9%–13.9% and 5.5%–14.9%, respectively.     

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC₅₀) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%–84.60%, with coefficients of variation of less than 12.2%.     

50Hz正弦磁场对细胞超氧产率及细胞增殖的影响

中国仓鼠肺成纤维细胞（CHL）和人脐静脉内皮细胞（HUVEC）受磁场辐照后,采用荧光光度法检测细胞的超氧产率和采用噻唑蓝比色分析法检测细胞的增殖情况。50Hz、0．4mT正弦磁场辐照30min引起CHL细胞和HUVEC细胞的超氧产率分别较对照组增加12％和19％：该磁场辐照24h导致CHL细胞和HUVEC细胞的数目分别较对照组上调9％和10％。提示50Hz、0.4mT正弦磁场促进CHL细胞和HUVEC细胞的增殖,可能与超氧自由基产率的上调有关。     

分光光度计有效测量范围的生化实验验证

为了在生物化学实验讲解中更直观地说明使用分光光度计时的有效测量范围,分别测定了不同浓度的苯丙氨酸和pSK质粒的光吸收值,与相应的标准浓度对照,计算出不同光吸收值的偏差,得到光吸收值与测量偏差的曲线。实验结果表明,当光吸收值小于0.1和大于1.0时,测量偏差超过10%,而当光吸收值在0.1~1.0之间时,测量偏差小于10%(当光吸收值在0.3~0.7之间时,测量偏差小于4%),测量值有效可信。本实验以氨基酸和核酸两种物质为例,验证了分光光度计的有效测量范围为0.1~1.0。     

聚乙烯醇的分子量及醇解度对其生物降解性的影响

从四川维尼纶厂生产车间下水道和污泥中分离出了可降解聚乙烯醇（PVA）的微生物Bacillus sp. 和Curtobacterium sp.，并建立了相应的PVA生物降解环境。采用吸光度分析方法考察了分子量及醇解度对PVA生物降解性的影响。结果表明：分离的微生物和以此为基础建立的生物降解环境可用于PVA生物降解性研究；在较短时间内，就可使PVA发生较高程度的降解；在所建立的降解环境中，PVA的分子量和醇解度对其降解性有一定的影响。降解前后FT-IR及1H NMR分析表明，在降解过程中PVA分子链发生了断裂。