全文获取类型
收费全文 | 1267篇 |
免费 | 27篇 |
国内免费 | 72篇 |
专业分类
教育 | 993篇 |
科学研究 | 179篇 |
体育 | 71篇 |
综合类 | 111篇 |
文化理论 | 2篇 |
信息传播 | 10篇 |
出版年
2023年 | 4篇 |
2022年 | 17篇 |
2021年 | 19篇 |
2020年 | 34篇 |
2019年 | 18篇 |
2018年 | 20篇 |
2017年 | 12篇 |
2016年 | 14篇 |
2015年 | 34篇 |
2014年 | 81篇 |
2013年 | 70篇 |
2012年 | 94篇 |
2011年 | 113篇 |
2010年 | 64篇 |
2009年 | 57篇 |
2008年 | 88篇 |
2007年 | 98篇 |
2006年 | 102篇 |
2005年 | 84篇 |
2004年 | 66篇 |
2003年 | 63篇 |
2002年 | 49篇 |
2001年 | 41篇 |
2000年 | 30篇 |
1999年 | 22篇 |
1998年 | 13篇 |
1997年 | 12篇 |
1996年 | 11篇 |
1995年 | 10篇 |
1994年 | 9篇 |
1993年 | 5篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1988年 | 1篇 |
排序方式: 共有1366条查询结果,搜索用时 15 毫秒
1.
对土家族城镇中小学生体表面积调查研究 总被引:4,自引:0,他引:4
为了解和建立土家族中小学生这方面的形态学指标,采用适合中国人的Stevenson标准公式,对土家族中小学生的体表面积进行了调查研究.结果显示:1)土家族中小学生体表面积值随着年龄增长而增长,且其增长速度男女生均可分为匀速增长期、快速增长期、速度减缓期3个不同时期;2)男女生体表面积曲线出现双交叉现象,即11岁时女生体表面积值超过男生,14岁时男生的体表面积又超过女生,15岁以后二者体表面积值的差异逐渐增大,表现出显著性差异. 相似文献
2.
基于点云数据的复杂型面实体建模研究 总被引:1,自引:1,他引:0
陈雪芳 《苏州市职业大学学报》2006,17(2):64-66
文章针对复杂型面产品的特点,从工业产品设计的实际应用出发,提出将正向设计的尺寸和定位关系设计方法和逆向设计的复杂曲面设计方法有效结合起来,基于点云数据实现产品的实体模型重建。以摩托车覆盖件为例,利用逆向工程软件从点云数据中提取和重构特征曲线与曲面,然后将这些几何元素信息在正向设计系统中结合特征建模方法创建整体三维CAD模型。 相似文献
3.
Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar A Al-Hartomy Swelm Wageh Abdullah G Al-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang 《国家科学评论(英文版)》2022,9(8)
The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-CoV-2 variants, including Omicron, are required to be identified as soon as the samples are identified as ‘positive’. Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time, we have developed MOPCS (Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance (SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat (CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-system-empowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification, within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-CoV-2, and mutations appear in variants B.1.617.2 (Delta), B.1.1.529 (Omicron) and BA.1 (a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations. 相似文献
4.
BackgroundPlanctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media achieves only low biomass yields, hindering research on these species. We therefore developed a chemically defined medium for the model organism Planctopirus limnophila to increase biomass production.ResultsWe found that P. limnophila grows best with a 10 mM sodium phosphate buffer. The replacement of complex nitrogen sources with defined amino acid solutions did not inhibit growth. Screening for vitamin requirements revealed that only cyanocobalamin (B12) is needed for growth. We used response surface methodology to optimize the medium, resulting in concentrations of 10 g/L glucose, 34 mL/L Hutner’s basal salts, 23.18 mM KNO3, 2.318 mM NH4Cl and 0.02 mg/L cyanocobalamin. The analysis of amino acid consumption allowed us to develop a customized amino acid solution lacking six of the amino acids present in Aminoplasmal 10%. Fed-batch cultivation in a bioreactor using the optimized medium achieved a final ΔOD600 of 46.8 ± 0.5 after 108 h, corresponding to a cell dry weight of 13.6 ± 0.7 g/L.ConclusionsThe optimized chemically defined medium allowed us to produce larger amounts of biomass more quickly than reported in earlier studies. Further research should focus on triggering P. limnophila biofilm formation to activate the gene clusters responsible for secondary metabolism.How to cite: Kruppa OC, Gerlach D, Fan R, et al. Development of a chemically defined medium for Planctopirus limnophila to increase biomass production. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.09.002. 相似文献
5.
王艳华 《桂林师范高等专科学校学报》2011,25(2):107-110
首先从情绪工作的定义、特征出发分析教师是属于高情绪工作者,之后阐述情绪工作的调节策略以及影响调节策略的相关因素,以期为研究教师职业提供一个新视角。 相似文献
6.
Jennifer Ward Stephanie Branson Megan D. Cross Ilene R. Berson 《Journal of Research on Technology in Education》2016,48(3):227-238
This study explored how developmentally appropriate practices influenced the affordances of a multitouch surface in an early childhood classroom. Children, ages 4 to 5 years old, were videotaped while engaged in a storymaking application on a multitouch table. As a result of the study, we found that some challenges with the functionality undermined children's autonomous control. The researchers observed numerous instances of issues with control of the medium, including height of the table, sensitivity to multiple types of touch, and limited instructions and prompts. Implications for future research and practice are discussed. 相似文献
7.
Ramesh Swaminathan Jonathan M. Williams Michael D. Jones 《Journal of sports sciences》2016,34(11):1058-1066
Artificial surfaces are now an established alternative to grass (natural) surfaces in rugby union. Little is known, however, about their potential to reduce injury. This study characterises the spinal kinematics of rugby union hookers during scrummaging on third-generation synthetic (3G) and natural pitches. The spine was sectioned into five segments, with inertial sensors providing three-dimensional kinematic data sampled at 40 Hz/sensor. Twenty-two adult, male community club and university-level hookers were recruited. An equal number were analysed whilst scrummaging on natural or synthetic turf. Players scrummaging on synthetic turf demonstrated less angular velocity in the lower thoracic spine for right and left lateral bending and right rotation. The general reduction in the range of motion and velocities, extrapolated over a prolonged playing career, may mean that the synthetic turf could result in fewer degenerative injuries. It should be noted, however, that this conclusion considers only the scrummaging scenario. 相似文献
8.
表面活性剂增敏光度法测定水样中的微量铁 总被引:4,自引:0,他引:4
梁国柱 《南通职业大学学报》2005,19(2):29-31
在HAc-NaAc缓冲溶液(pH=5)存在下,以邻二氮菲(phen)作显色剂,表面活性剂曲拉通X-100对Fe(II)与邻二氮菲的显色反应具有增敏作用,形成的Fe(II)-phen-Triton三元络合物呈红色,络合物的最大吸收波长为510 nm,表观摩尔吸光系数为1.2×105L.mol-1.cm-1,铁含量在0.5~12μg/mL范围内符合比尔定律,用于水样中微量铁的测定,回收率在97.1%~98.0%之间。 相似文献
9.
介绍了Mathematica在几种几何图形绘制中的应用,提出了获得空间旋转曲面的参数方程的一般方法,并给出了用Mathematica绘制空间旋转曲面的实例的源代码. 相似文献
10.
黄海 《深圳职业技术学院学报》2007,6(1):8-10
介绍了实际生产过程中加工内扁椭球面时,求算合适铣刀半径的问题,通过推导,给出了求算最大铣刀半径公式和推荐适当的系数,以便机床操作者和工程技术人员参考选用。 相似文献