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卵黄抗体(Ig Y)是指用特定的抗原免疫产蛋鸡后,从免疫鸡蛋中获得的针对免疫抗原产生的特异性抗体。其制作简单,价格相对低廉,在疾病诊断、治疗、预防等方面有着广泛的应用。本文就卵黄抗体在病毒性疾病预防、治疗与检测中的应用进行相关的探讨和分析。 相似文献
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本文应用光学、电子显微镜技术研究栉孔扇贝(Chlamysfarreri)卵子发生过程中卵细胞内部构造的变化.根据细胞大小、卵黄粒的有无、核仁的形态、细胞器演变特点,栉孔扇贝的卵子发生可分为卵原细胞、卵黄发生前期的和卵黄发生期的卵母细胞三个时期. 相似文献
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鸡禽流感病原分离鉴定及高免卵黄抗体研制 总被引:1,自引:0,他引:1
从出现呼吸道症着状和产蛋量下降的产蛋鸡群的气管分泌物中分离病毒,经HA活性测定,分离尿囊液中新城疫病毒排除试验,禽流感A型特异性血清的琼脂扩散实验、A型流感病毒的亚型鉴定试验,分离到的病毒为H9N2禽流感病毒,用禽流感H9N2亚型油乳剂灭活苗多次免疫产蛋鸡,获得高希蛋,并制成禽流感高免卵黄液,在发病初期给禽流感病鸡肌注高免卵黄流,获得了满意的效果。 相似文献
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对人教版高中《生物》选修3中“哺乳动物受精阶段”的教学内容,通过一轮教学实践后,我发现,要想让学生全面深刻掌握“受精阶段”的话,除了要对放射冠、卵丘、卵黄膜、微绒毛几个知识有所复习解释外,还应注意以下四个方面: 相似文献
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实验用鸡IBD以及幼儿风疹病毒连续免疫产卵母鸡3次,制备出高免卵黄抗体,并对免疫球蛋白的含量进行检测。结合琼脂双扩测定免疫球蛋白效价为:1:64-1:128,风疹抗体ELISA法测定效价:1:10204-1:20480的结果,表明本实验的免疫程序是可行的。结合鸡的其他一些生物学特点,表明利用免疫鸡制备抗体是一种简便、经济、高效的抗体制备方法。 相似文献
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Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk 总被引:3,自引:0,他引:3
Specific immunoglobulin (IgY) from egg yolk against Aeromonas hydrophila was produced by immunization of White Leghorn hens with formalin-killed whole cells of A. hydrophila. ELISA test using A. hydrophila as the coating antigen revealed that the specific antibody titer started to increase in the egg yolk at the 13th day post-immunization (P/N=2.18), reached the peak at the 56th day (P/N= 13.82), and remained at high level until day 133 (P/N=7.03). The antibody was purified by saturated ammonium sulphate with a recovery rate of 63.5%. The specific IgY inhibited the growth ofA. hydrophila at a concentration of 1.0 mg/ml during the 18 h incubation. Pre-treatment of polyploid gibel carps Carassius auratus Gibelio with specific IgY had a protection rate of 60% (6/10) against challenge with A. hydrophila, while none of the fishes in the control groups receiving sterile phosphate buffered saline (PBS) or non-specific IgY survived the challenge. Treatment of fishes with the specific IgY 4 h after the challenge also had lower mortality (70%, 7/10), a 30% reduction against the control PBS or non-specific IgY groups (10/10). These results indicate that specific IgY antibodies could be obtained easily from hens immunized with an inactivated A. hydrophila and could provide a novel alternative approach to control of diseases in fishes caused by this organism. 相似文献
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笔者研制的双价高免卵黄抗体经保护试验证实,传染性法氏囊病(IBD)和新城疫(ND)的预防保护率为100%和93.3%,治疗试验的治愈率分别为90%和87.5%,经过各地实地应用,效果明显. 相似文献
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魏向阳 《赤峰学院学报(自然科学版)》2006,(6)
肉用种鸡急性大肠杆菌病在养殖生产中时有出现,给养殖户造成巨大损失.本文根据作者多年临床经验,结合一例典型病例展开阐述,针对肉用种鸡急性大肠杆菌的检测、诊断、预防及治疗,提出了一整套切实可行的措施,对当前本地肉鸡生产将产生一定的推动作用. 相似文献