运动对RBP4诱导的脂代谢异常鼠肝脏ACC1 表达和脂肪合成的影响

目的:研究运动对RBP4诱导的脂代谢异常鼠肝脏ACC1表达和脂肪合成的影响.方法:重组人RBP4注射建立脂代谢异常鼠模型.设一次性运动组(RBP4 One-time exercise group,ROE 组)、8周有氧运动组(RBP4 aerobic exercise group,RAE组)和安静对照组(RBP4 control group,RC组).结果:ROE组血清RBP4水平与RC组相比显著降低(P＜0.05),血清TG和肝脏TG含量无显著改变(P＞0.05).RAE组血清RBP4、TG和肝脏TG含量与RC组和ROE组相比均显著降低(P＜0.01).与RC组相比,ROE组和RAE组肝脏ACC1mRNA表达均显著降低(P＜0.01).与RC组相比,ROE组ACC1蛋白表达无显著改变(P＞0.05).与RC组和ROE组相比,RAE组ACC1蛋白表达均显著降低(P＜0.01).结论:高RBP4显著增加小鼠肝脏ACC1基因和蛋白表达增加,促进肝脏脂肪合成.8周有氧运动抑制了RBP4在肝脏的脂肪合成促进作用,改善脂代谢,机制涉及降低RBP4水平、减少肝脏ACC1基因和蛋白表达以及TG的合成.     

运动对肥胖状态下LKB1-AMPK-ACC信号传导通路的影响

肥胖已经成为危险人类健康的常见慢性疾病,而运动是目前公认的治疗肥胖最为有效的方法之一。AMPK(腺苷酸活化蛋白激酶)是一种比较重要的代谢性应激蛋白激酶,在全身的能量平衡中起着总开关的作用。LKB1可以活化细胞中的AMPK,AMPK使ACC(乙酰辅酶A羧化酶)磷酸化失活,脂肪酸合成减少、氧化分解增加,从而预防肥胖的发生发展。肥胖状态下,AMPK磷酸化水平降低,ACC活性增强,脂肪酸合成增加、氧化速率下降。而运动是改善肥胖的重要手段,可以使AMPK磷酸化增强,ACC活性减弱。主要阐述肥胖状态下LKB1-AMPK-ACC信号传导通路各级联蛋白的变化趋势,以及运动干预对各级联蛋白含量的影响。     

综合测试题型网上考试的技术实现

本文介绍用VB、ASP和ACCESS编程,实现网上测验和考试的方法、关键程序,为远程开放教育的网上考试提供一个平台。     

零化子特殊升链条件与内射性

给出满足右零化子特殊升链条件的环是QF环的若干条件。主要证明左、右自内射环,左IF环,右IF右P-内射环且满足右零化子特殊升链条件,则它是QF环。     

Model predictive control for adaptive cruise control with multi-objectives: comfort, fuel-economy, safety and car-following

For automated vehicles,comfortable driving will improve passengers’ satisfaction.Reducing fuel consumption brings economic profits for car owners,decreases the impact on the environment and increases energy sustainability.In addition to comfort and fuel-economy,automated vehicles also have the basic requirements of safety and car-following.For this purpose,an adaptive cruise control (ACC) algorithm with multi-objectives is proposed based on a model predictive control (MPC) framework.In the proposed ACC algorithm,safety is guaranteed by constraining the inter-distance within a safe range; the requirements of comfort and car-following are considered to be the performance criteria and some optimal reference trajectories are introduced to increase fuel-economy.The performances of the proposed ACC algorithm are simulated and analyzed in five representative traffic scenarios and multiple experiments.The results show that not only are safety and car-following objectives satisfied,but also driving comfort and fuel-economy are improved significantly.     

Determining motions with an IMU during level walking and slope and stair walking

ABSTRACT

This study investigated whether using an inertial measurement unit (IMU) can identify different walking conditions, including level walking (LW), descent (DC) and ascent (AC) slope walking as well as downstairs (DS) and upstairs (US) walking. Thirty healthy participants performed walking under five conditions. The IMU was stabilised on the exterior of the left shoe. The data from IMU were used to establish a customised prediction model by cut point and a prediction model by using deep learning method. The accuracy of both prediction models was evaluated. The customised prediction model combining the angular velocity of dorsi–plantar flexion in the heel-strike (HS) and toe-off (TO) phases can distinctly determine real conditions during DC and AC slope, DS, and LW (accuracy: 86.7–96.7%) except for US walking (accuracy: 60.0%). The prediction model established by deep learning using the data of three-axis acceleration and three-axis gyroscopes can also distinctly identify DS, US, and LW with 90.2–90.7% accuracy and 84.8% and 82.4% accuracy for DC and AC slope walking, respectively. In conclusion, inertial measurement units can be used to identify walking patterns under different conditions such as slopes and stairs with customised prediction model and deep learning prediction model.     

Estrogen receptor expression in adrenocortical carcinoma

Objective: Adrenocortical carcinoma (ACC) is a rare but highly malignant tumor, and its diagnosis is mostly delayed and prognosis is poor. We report estrogen receptor (ER) expression in this tumor and our clinical experiences with 17 ACC cases. Methods: The data of the 17 patients (9 females and 8 males, age range from 16 to 69 years, mean age of 42.6 years) with ACC were reviewed, and symptoms, diagnostic procedures, treatment, and results of follow-up were evaluated. Immunohistochemistry was used to detect ER expression in tumor samples from the 17 patients. Results: At the time of diagnosis, 4 tumors were classified as Stage Ⅰ, 4 as Stage Ⅱ, 3 as Stage Ⅲ, and 6 as Stage Ⅳ. Eight patients demonstrated positive nuclear immunostaining of ER. The prognosis of patients with ER positive was significantly better (P<0.05) than that of patients with ER negative, with 1- and 5-year survival rates at 86% and 60% for ER-positive patients, and 38% and 0% for ER-negative patients, respectively. Conclusion: ER-positivity may be one of the factors associated with a worse prognosis of ACC.     

番茄ACC合成酶反义基因在转基因猕猴桃中表达的初步研究

利用从番茄(Lycopersicum esculentumMill.)果实中分离到的ACC合成酶cDNA基因，反向置于CaMV35S启动子的控制之下(所用根癌杆农菌中合有改建后分别携带嵌合NPT Ⅱ基因和番茄的ACC合成酶反义基因的质粒)，并转入美味猕猴桃(Actinidiadeliciosa cv.Hayward)的愈伤组织中。通过分化，2周后获得了能在附加羧苄青霉素(Carbenicillin,500mg/l)和卡那霉素(Kanamycin,50mg/l)的MSA(MS盐类，3．0mg/lBA，0．2mg/l IAA)培养基上生长的抗性愈伤组织。经过半年多的继代培养并筛选，获得了大量的抗卡那霉素(50mg/l)的丛芽以及由抗性芽发育形成的抗性试管苗。探讨了在用卡那霉素进行筛选的条件下影响愈伤组织诱导率的一些因素。     

老年急性非结石性胆囊炎临床特点及手术治疗

目的:探讨老年人急性非结石性胆囊炎(AAC)临床特点与手术治疗.方法:通过设立病例对照,对48例老年人AAC和急性结石性胆囊炎(ACC)的并发症、胆囊病理改变及术后结果进行比较.结果:与老年人ACC相比,AAC患者以心血管疾病为主的并发症明显增多(P＜0.01);胆囊化脓、坏疽与穿孔率明显增高(P值＜0.01,＜0.01,＜0.05);术后并发症发生率明显上升(P＜0.01).结论:老年人AAC并存病多、胆囊病变重、术后并发症发生率高;宜在症状出现48h内手术治疗.