TSH Comparison Between Chemiluminescence (Architect) and Electrochemiluminescence (Cobas) Immunoassays: An Indian Population Perspective

Although 3rd generation TSH assays are the most widely used immunoassays, credible comparison studies, specially involving Indian sub-populations are practically non-existent. To compare the TSH measurements between chemiluminescence (Architect) and electrochemiluminescence (Cobas) inmmunoassays in an urban ambulatory Indian population. 1,615 subjects were selected randomly from the usual laboratory workflow, their TSH measured in Architect and Cobas and the paired data thus generated were statistically analysed. TSH values of Cobas were observed to be higher than the Architect values by 28.7 %, with a significant proportional difference between the two, but majority of the Cobas values (above 90 %) were within the limits of agreement with Architect values. In situations where both the instruments are in use simultaneously, a standardization of the methods is imperative, in larger interest of the patient populace.     

自发电池激发的电化学发光分析

本文简单介绍了电化学发光分析近年来的研究进展.目前,文献报道的ECL分析都是使用外加的电源来实现ECL的激发,这就限制了ECL检测系统的微型化,及其在微全分析系统中的进一步应用.我们曾经设计了微型自发原电池为鲁米诺体系及钙黄绿素的电化学发光提供激发电位,大大地简化了ECL的设备,在ECL系统的微型化方面,迈出了新的步伐.     

电化学发光法测定碘的研究

在流通体系中,于电解池的三电极系统的工作电极铂片上施加0.35v(相对于AgCl/Ag电极)的电势,使KCl-Na2CO3-NaHCO3-I-Luminol电解液中I-氧化成单质I2. 生成的I2与Luminol反应,产生化学发光. 电化学发光强度与I-浓度成正比,据此测定I-浓度,本法选择性较好,检出限为4.1×10-10g/mL,线性范围为1.2×10-7～1.0×10-9g/ml,对1.0×10-7g/ml的I-七次平行测定的相对偏差为2.8%,成功地用于食盐中碘的测定.