胃镜污染HBV、HCV和HIV的调查

目的　检测胃镜常规清洗消毒后HBV、HCV、HIV污染残留状况.方法　随机取样.巢式聚合酶链反应法检测HBV DNA,逆转录巢式聚合酶链反应法检测HCV RNA、HIV RNA,放免法检测HBsAg.结果　对605例标本进行检测,未检出阳性标本.结论　胃镜经常规消毒后未检到HBV、HCV和HIV的污染,但进一步规范胃镜消毒操作方法,提高消毒质量,意义重大.     

我校体育系学生乙肝表面抗原携带情况调查

调查体育系学生HBsAg阳性率 17.1%高于全国平均水平 .目的是为了更有效地防治乙肝病毒的传播 ,有益于学生的身心健康 .     

HBV携带者家庭内感染因素的调查分析

从HBV携带者生活的年限、就餐方式、与家庭成员之间的关系、家庭成员中各车龄组的感染率四个方面进行观察,结果表明家庭内乙型肝炎水平传播的主要暴露因素有日常生活密切接触,家庭未实行分餐吃饭和夫妻性生活,提出了控制家庭内感染的防制措施．     

乙肝歧视的成因与救济

乙肝弱势人群所受到的歧视主要来自于社会的封闭、人们观念的误区以及隐藏在其后的功利主义观。要消除乙肝歧视。必须建立立法、行政、司法三位一体的救济体系。     

HBV核酸光敏生物素探针的制备

利用设计合成的特异性引物，通过PCR扩增获得乙肝病毒的S区621个bpDNA片段．该扩增产物经EcoRI与BamHI双酶切后直接克隆进入PSK载体进行表达，再通过扩增重组菌株，以获得大量的S区DNA片段，对它们进行光敏生物素标记，制成探针，对乙肝病毒进行检测灵敏度高，特异性强，其灵敏度可达到4pg水平。     

Science Letters： Solid lipid nanoparticles loading adefovir dipivoxil for antiviral therapy

Herein, solid lipid nanoparticles (SLN) were proposed as a new drug delivery system for adefovir dipivoxil (ADV). The octadecylamine-fluorescein isothiocynate (ODA-FITC) was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15. The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system. About 15wt% drug entrapment efficiency (EE) and 3wt% drug loading (DL) could be reached in SLN loading ADV. Comparing with free ADV, the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA levels in vitro were significantly enhanced.     

郴州师专1997-1999年新生HBsAg检测结果分析

通过检测 1997～ 1999年郴州师专入学新生乙肝表面抗原 (HBsAg) ,发现其总阳性率为 13.6 8% ,高于我国人群HBsAg平均阳性率 8.83% ,其中男生阳性率明显高于女生 ;进一步研究发现男、女HBsAg阳性率存在显蓍差异的主要原因是男生使用未消毒公用餐具就餐的频率明显高于女生 ,而与性别并无直接关系 .研究表明HBsAg阳性率与使用未消毒公用餐具频率之间的相关系数r=0 .84.     

临床145份血清标志HBsAg,HBeAg,Anti－HBc与HBV－DNA的检测结果分析

本通过145份乙型肝炎血清标本免疫测定乙肝病毒抗原抗体及PCR检测结果进行统计分析，研究两种测定结果的临床意义及相关性。     

Metabonomic analysis of hepatitis B virus-induced liver failure: identification of potential diagnostic biomarkers by fuzzy support vector machine

Hepatitis B virus (HBV)-induced liver failure is an emergent liver disease leading to high mortality. The severity of liver failure may be reflected by the profile of some metabolites. This study assessed the potential of using metabolites as biomarkers for liver failure by identifying metabolites with good discriminative performance for its phenotype. The serum samples from 24 HBV-induced liver failure patients and 23 healthy volunteers were collected and analyzed by gas chromatography-mass spectrometry (GC-MS) to generate metabolite profiles. The 24 patients were further grouped into two classes according to the severity of liver failure. Twenty-five commensal peaks in all metabolite profiles were extracted, and the relative area values of these peaks were used as features for each sample. Three algorithms, F-test, k-nearest neighbor (KNN) and fuzzy support vector machine (FSVM) combined with exhaustive search (ES), were employed to identify a subset of metabolites (biomarkers) that best predict liver failure. Based on the achieved experimental dataset, 93.62% predictive accuracy by 6 features was selected with FSVM-ES and three key metabolites, glyceric acid, cis-aconitic acid and citric acid, are identified as potential diagnostic biomarkers.     

大学新生乙型肝炎病毒感染调查

本文报道近三年安徽农业技术师范学院新生动型肝炎病毒感染调查结果。受检学生1628人，乙型肝炎表面抗原阳性率为10～12％，谷丙转氨酶异常率为4～6％，西指标均异常为0．9～1．5％。