扁藻细胞电镜观察的制样技术

以海洋单细胞微藻亚心型扁藻为材料 ,对海水水样中活体单细胞微藻的亚显微结构观察样品的制备技术进行了探索。选用 2 .5 %的戊二醛固定液和 1 %的锇酸固定液对样品进行双固定的技术 ;先酒精后丙酮按浓度梯度顺次进行脱水 ;再用Epon81 2环氧树脂作为包埋剂进行包埋 ;最后经过醋酸铀 -枸橼酸铅双染色后将切片用透射电镜进行亚显微结构的形态观察。采用本方法进行水样中活体样品的电镜制片 ,拍得清晰度较高的电镜照片 ,并通过照片对水样中运动细胞透射电镜样品制备技术进行了分析。     

针刺和静力牵张对大负荷运动后骨骼肌M线变化影响的免疫电镜研究

用胶体金标记技术定位运动后及运动后经针刺或静力牵张处理的人骨骼肌M蛋白,以探讨超过习惯负荷后骨骼肌收缩结构变化以及针刺和静力牵张对促进其恢复的机制。我们发现:大负荷斜蹲后未经针刺的对照腿股外肌M线的M蛋白标记密度低于针刺腿(P<0.05),而对照腿M线两侧及M线两侧以外的M蛋白标记密度高于针刺腿(P<0.05)。对照实验证明运动腿M线以外增加的标记物为物异性标记。静力牵张组在M线及M线两侧的标记结果与针刺组一致。仅在M线两侧以外区域牵张腿与运动对照腿之间标记密度的差异没有统计学意义。我们还观察到运动后对照腿变化M线体密度达42—48％,而针刺或牵张腿为6—9％。上述结果表明大负荷运动加强了负荷后骨骼肌M蛋白的解聚或降解,从而导致M线结构改变;针刺和静力牵张可显著促进M蛋白的合成或抑制其降解,而使M线结构恢复或稳定。本文还探讨了M线变化与肌粗丝结构变化的关系。     

Seeking Nettle Textiles – Utilizing a Combination of Microscopic Methods for Fibre Identification

Bast fibres have been commonly used as a textile material in Northern Europe since Neolithic times. However, the process of identifying the different species has been problematic, and many important questions related to their cultural history are still unanswered. For example, a modified Herzog test and the presence of calcium oxalate crystals have both been used in identification. In order to generate more reliable results, further research and advancement in multi-methodological methods is required. This paper introduces a combination of methods which can be used to identify and distinguish flax (Linum usitatissimum), hemp (Cannabis sativa), and stinging nettle (Urtica dioica). The research material consisted of reference fibres and 25 fibre samples obtained from 12 textiles assumed to be made of nettle. The textiles were from the Finno-Ugric and Historical Collections of The National Museum of Finland. The fibre samples were studied by observing the surface characteristics and cross sections with transmitted light microscopy, and by using a modified Herzog test with polarized light, in order to identify the distinguishable features in their morphological structures. The study showed that five out of 25 samples were cotton, 16 nettle, one flax, and one hemp. Findings from two samples were inconsistent. The results show that it is possible to distinguish common north European bast fibres from each other by using a combination of microscopic methods. Furthermore, by utilizing these combined methods, new and more reliable information could be obtained from historical ethnographic textiles, which creates new vistas for the interpretation of their cultural history.     

Virtual Microscopy as a Learning Tool in Brazilian Medical Education

Virtual microscopy (VM) is a widely used teaching method in Medical Education in many developed countries. In Brazil, however, this is not the case for most medical schools, considering Brazilian social inequality and uneven access to technology. Recently, the Covid-19 pandemic has also challenged Universities to seek and make a transition toward more effective methods of full-time online education. Thus, the main goal of this work was to verify student's perception and academic performance, assessed upon VM implementation in a Brazilian Medical School. Ribeirao Preto Medical School students answered a 26-question survey with regards to optical microscopy (OM) and VM. Academic performance was compared between participants that were (year of 2019) or were not (year of 2015) exposed to VM. Taken the results together, subjective impressions such as handling, suitability, learning effectiveness, and pleasure using the tools, have shown a higher score for virtual microscopy (median = 29), when compared to optical microscopy (median = 24) with a P-value < 0.001 by Wilcoxon rank test, upon measurement using an ordinal scale. Regarding academic performance, no statistically significant differences were found between groups (P-value = 0.38, Cohen's d = 0.19). Therefore, VM proved to be adequate to the Brazilian medical education in light of Brazilian social contexts and Covid-19 pandemic.     

三甲基铝双聚体的结构和键合作用

以杂化理论、闭合的三中心两电子键模型对Al2 (CH3 ) 6之键角、键长等作出合理的解释 ,明确Al2 (CH3 ) 6的空间构型     

拓扑指数mP在价电子轨道能量中的相关性研究

本文提出了一种新的键参数拓扑指数^mP.用其零、一阶指数0p、1p分别与20种碱金属卤化物的晶格能U、生成焓△Hf,m,离子水化能△Gh,m和核间距R0以及16种碱金属卤化物的熔点Tm、磁化率xm和键长Bd的相关联,采用最小二乘法,拟合的回归方程的相关系数分别为0.995、0.988、0.996、0.995、0.964、0.988、0.964,满足优级标准,预测结果良好.     

电子类教学仪器的抗振防振设计

对电子教学设备的抗振从理论和实践上进行了探讨，并介绍了电子类教学设备的抗振设计原则和方法。     

影响我国电子商务发展的十大因素

电子商务作为一种新的经济运行模式,将成为21世纪信息世界的核心。而基础设施建设、电子交付、安全、法律、人才、政府参与、观念意识、信用经济制度建设、企业信息化管理水平、物流现代化等十大因素却影响我国电子商务的发展。     

长光程薄层光谱电化学技术及应用

提供电子转移反应实验研究的实验装置及技术，依此可获得电子转移数、式电极电势、式异相电子转移速率常数、电子传递系数及还原态和氧化态的扩散系数等一系列重要的电化学参数。还可依此为基础设计出新颖、实用的教学实验。     

在羰基亲核加成反应活性中醛、酮的结构所起的作用

讨论了醛、酮结构在羰基亲核加成反应中所起的作用;还讨论了羰基的电子因素和空间因素;进一步用键角因素丰富和完善了传统理论,用许多因素解释了这一理论.