陇西栽培蒙古黄芪酯酶同工酶数量分析

为了对陇西栽培蒙古黄芪（Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao）的优质高产和良种选育提供一定依据,本文采用垂直板聚丙烯酰胺凝胶电泳技术,对该地区栽培蒙古黄芪种子酯酶同工酶进行了研究。结果表明：该地栽培蒙古黄芪是一个复杂的异质群体;种子酯酶同工酶电泳技术是检验蒙古黄芪种内变异的一种有效手段。     

Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction

Astragalus mongholicus (AM) derived from the dry root of Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is a widely used traditional Chinese medicine. The present study investigated the potential role of AM on renal fibrosis on a rat model of unilateral ureteral obstruction (UUO). We divided 48 Sprague-Dawley rats randomly into 4 groups: sham-operated group (Sham), untreated UUO group, AM-treated (10 g/(kg.d)) UUO group, and losartan-treated (20 mg/(kg.d)) UUO group as positive control. Haematoxylin & eosin (HE) and Masson staining were used to study the dynamic histological changes of the kidneys 7 and 14 d after operation. The expressions of fibronectin (FN), type I collagen (coII), hepatocyte growth factor (HGF), transforming growth factor-pi (TGF-βl), and a-smooth muscle actin (a-SMA) were analyzed by real-time polymerase chain reaction (PCR), immunohistochemistry staining, and Western blot. Results show that, similar to losartan, AM alleviated the renal damage and decreased the deposition of FN and colI from UUO by reducing the expressions of TGF-pi and a-SMA (P<0.05), whereas HGF increased greatly with AM treatment (P<0.05). Our findings reveal that AM could retard the progression of renal fibrosis. The renoprotective effect of AM might be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-β1 expression.     

陇西栽培蒙古黄芪生物学性状的多元统计分析

为了探测陇西栽培蒙古黄芪的表型多样性及自然类型,对该地栽培蒙古黄芪的11个主要生物学性状进行了主成分分析和Q型聚类分析. 多元统计分析表明,陇西栽培蒙古黄芪在生物学性状上存在较大的变异,尤其在果荚颜色、种子花纹及茎色等性状上显著分化,形成了红色类型、红荚绿茎类型和绿色类型等3个主要蒙古黄芪自然类型,因而该地栽培的蒙古黄芪是一个复杂的异质群体.     

五种黄芪属植物的核型分析

The karyotypes of five species in Astragalus (A. dahuricus DC., A. mongolicus Bunge., A. adsurgens Pall., A. melilotoides Pell., A. huangheensis H. C. Fu., Y. H. Liu) were studied.  Among them, the karyotypes of A. dahuricus and A. melilotoides are re- ported for the first time.  While A. melilotoides is tetraploid (2n=4X=32), all the others are found to be diploid (2n= 2X = 16).  Based on the comparison of karyotypes, the evolu-tionary order of these 5 species is discussed.     

陇西栽培蒙古黄芪酯酶同工酶数量分析

为了对陇西栽培蒙古黄芪（Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao）的优质高产和良种选育提供一定依据，本文采用垂直板聚丙烯酰胺凝胶电泳技术，对该地区栽培蒙古黄芪种子酯酶同工酶进行了研究。结果表明：该地栽培蒙古黄芪是一个复杂的异质群体；种子酯酶同工酶电泳技术是检验蒙古黄芪种内变异的一种有效手段。     

黄芪中药种衣剂对黄芪的包衣效果研究

主要发生在苗期的根腐病以及花荚期的蚜虫病,是黄芪生长发育过程中经常发生的病虫害,本文应用课题组自制的种衣剂对黄芪种子进行包衣,不包衣的黄芪种子为对照组,通过田间试验,以黄芪种子发芽率、黄芪根腐病发生情况、蚜虫发生程度三者为考察对象,从而针对自制黄芪中药种衣剂对黄芪的包衣效果进行初步的研究。