水分胁迫下亚精胺对小麦幼苗蛋白质含量与蛋白酶活性的影响

研究了水分胁迫下亚精胺对小麦幼苗相对含水量、叶绿素总量、蛋白质含量和蛋白酶活性的影响。结果显示,亚精胺预处理延缓水分胁迫下小麦幼苗叶片含水量、叶绿素含量和蛋白质含量的下降,抑制蛋白酶活性的上升。表明亚精胺预处理对水分胁迫的小麦幼苗具有防衰抗旱的保护作用。     

亚精胺、低N素及昼夜温度变化诱导离体黄瓜子叶花芽分化

试验了亚精胺(Spd)与激动素(KT)复合处理、不同的N素及昼夜温度变化对离体黄瓜子叶花芽分化的影响。发现:KT 9.3×10-3mm o l.L-1 Spd5×1-0 2mm o l.L-1有利于花芽的诱导,低N素和昼夜变化促进花器官的构建,进一步完善了离体黄瓜子叶花芽分化的试验体系。     

外源亚精胺对黄瓜种子萌发及再生植株生长发育的影响

以黄瓜为试材,研究了种子在含不同浓度亚精胺的MS培养基上的萌发情况,然后将处理后的七龄幼苗去3／4下胚后转接到不含亚精胺的MS培养基中,统计再生植株的株高、节间平均长度、叶面积、雌株（长有雌花再生植株）比率、雌花总数．结果表明,较低浓度的亚精胺显著促进黄瓜种子的萌发且有利于再生植株生殖生长,显著提高雌花成花率;而较高浓的亚精胺则对种子的萌发表现出明显的抑制作用,也不利于再生植株叶片生长和花芽分化,但促进节间伸长．     

渗透胁迫下小麦幼苗叶片亚精胺和抗氧化非酶物质的关系

研究了渗透胁迫下,小麦品种豫麦18(抗旱性较强)和扬麦9号(抗旱性较弱)幼苗叶片中亚精胺(Spd)和抗氧化非酶物质含量的变化.渗透胁迫2 d,抗旱性弱的扬麦9号小麦幼苗叶片的过氧化氢(H2O2)和丙二醛(MDA)含量的上升幅度明显大于抗旱性强的豫麦18,而豫麦18幼苗叶片Spd、抗坏血酸(ASA)和谷胱甘肽(GSH)含量的上升幅度明显大于扬麦9号.胁迫条件下,外源Spd处理扬麦9号,不仅明显提高其幼苗叶片的Spd含量,而且ASA和GSH含量也明显提高,而其H2O2和MDA则明显下降;Spd的生物合成专一性抑制剂——甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理豫麦18,则明显抑制其在渗透胁迫下叶片中Spd,ASA和GSH含量的上升,而明显促进其H2O2和MDA含量的上升.这些结果表明,渗透胁迫下小麦幼苗叶片中的Spd减轻活性氧对幼苗伤害的原因之一,可能是通过提高ASA和GSH等抗氧化非酶物质含量而降低活性氧水平.     

Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes

Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against the peroxidative action of H₂O₂ which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H₂O₂ and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines may have independent mechanism of action.