Exercise training increases the S-Klotho plasma levels in sedentary middle-aged adults: A randomised controlled trial. The FIT-AGEING study

ABSTRACT

This study aimed to investigate the effects of different training modalities on the soluble Klotho (S-Klotho) plasma levels in sedentary middle-aged adults. A total of 74 middle-aged adults (53.4 ± 5.0 years old; 52.7% women) were enrolled in the FIT-AGEING study. We conducted a 12-week randomised controlled trial. The participants were randomly assigned to 4 different groups: (i) a control group (no exercise), (ii) a physical activity recommendation from the World Health Organization group (PAR), (iii) a high intensity interval training group (HIIT), and (iv) a high intensity interval training group adding whole-body electromyostimulation training group (HIIT-EMS). S-Klotho plasma levels, anthropometric measurements, and body composition variables were measured before and after the intervention programme. All exercise training modalities induced an increase in the S-Klotho plasma levels (all P ≤ 0.019) without statistical differences between them (all P ≥ 0.696). We found a positive association between changes in lean mass index and changes in the S-Klotho plasma levels, whereas a negative association was reported between changes in fat mass outcomes and changes in the S-Klotho plasma levels after our intervention study. In conclusion, our results suggest that the link between exercise training and the increase in S-Klotho plasma levels could be mediated by a decrease of fat mass and an increase of lean mass.     

Does moderate-intensity continuous training result in greater total energy expenditure compared to high-intensity interval training?

Recently in the Journal of Sports Sciences, Schaun et al. published a study on the comparison of energy expenditure between high-intensity interval training and moderate continuous training performed in water. With this Letter to the Editor, we would like to comment on the methodological aspects that should be considered to analyze the results presented, as well as the conclusions.

Abbreviations: CONT, moderate-intensity continuous training; EPOC, excess post-exercise oxygen consumption; HIIT, high-intensity interval training; VO2max, maximal oxygen consumption.     

HIIT与持续运动对成年人心肺功能影响的meta分析

分析高强度间歇运动(HIIT)与中等强度持续运动(CMT)对心肺功能的对比影响。方法:通过搜集相关数据库,知网、万方、百链、PubMed、Ebsco、Springer Link、Science Direct进行检索(到2018年9月),并设定研究纳入和剔除标准,提取各研究VO2max变化值(x±s),通过Rev Man 5. 3进行meta分析。结果:从1045篇文献筛选,最终28篇文献符合纳入标准。分析结果表明:HIIT与CMT比较,HIIT提高VO2max较明显(+1. 45mL·Kg^-1min^-1,95%CI:0. 86-2. 05,p<0. 01),具有统计学意义。结论:HIIT能有效的改善成年人的心肺功能,且相对于CMT是一种省时有效的改善成年人心肺功能方式。     

Energy expenditure and EPOC between water-based high-intensity interval training and moderate-intensity continuous training sessions in healthy women

The present study compared the energy expenditure (EE) during and after two water aerobics protocols, high-intensity interval training (HIIT) and moderate continuous training (CONT). A crossover randomized design was employed comprising 11 healthy young women. HIIT consisted of eight 20s bouts at 130% of the cadence associated with the maximal oxygen consumption (measured in the aquatic environment) with 10s passive rest. CONT corresponded to 30 min at a heart rate equivalent to 90–95% of the second ventilatory threshold. EE was measured during and 30 min before and after the protocols and excess post-exercise oxygen consumption (EPOC) was calculated. Total EE during session was higher in CONT (227.62 ± 31.69 kcal) compared to HIIT (39.91 ± 4.24 kcal), while EE per minute was greater in HIIT (9.98 ± 1.06 kcal) than in CONT (7.58 ± 1.07 kcal). Post-exercise EE (64.48 ± 3.50 vs. 63.65 ± 10.39 kcal) and EPOC (22.53 ± 4.98 vs.22.10 ± 8.00 kcal) were not different between HIIT and CONT, respectively. Additionally, oxygen uptake had already returned to baseline fifteen minutes post-exercise. These suggest that a water aerobics CONT session results in post-exercise EE and EPOC comparable to HIIT despite the latter supramaximal nature. Still, CONT results in higher total EE.     

高强度间歇训练(HIIT)对主观负荷强度和情绪体验影响的系统综述

目的:该文使用系统综述方法对关于高强度间歇训练对参与者主观负荷强度(RPE)以及情绪体验影响的现有实证研究主要结果进行整合与综述。方法:通过文献检索与筛查,共纳入22项符合条件的研究,对这些研究从研究设计、参与者特征、HIIT练习任务,以及HIIT对RPE和个体情绪体验影响的主要结果进行了系统分析。结果:(1)现有研究多从HIIT与中等强度连续训练(MICT)的对比研究角度入手;(2)研究被试相对较为单一,HIIT活动以"中量规范"任务为主;(3)HIIT活动中和活动后的RPE等级一般高于或等同于MICT组。(4)对练习的主观情绪检验也多为积极,但较强依赖于单次练习持续的时间。结论:作为一种较为复杂的锻炼活动形式,HIIT对主观负荷强度和情绪体验的影响与MICT相比更为复杂多样,其效果与HIIT任务规范、参与者身体条件等因素密切相关。在此基础上对今后研究亟需解决的问题及应注意的问题进行了讨论。     

发展HIIT改善大学生心肺功能的可行性分析

通过文献资料法分析近年来学生心肺功能下降的原因及严重性和HIIT的特点;运用Rev Man5.3进行meta分析验证HIIT对改善心肺功能的有效性。近年来国内外学者通过对HIIT的研究发现,HIIT对于改善心肺功能有其独特的优势,并结合现状分析发现可以通过发展HIIT来改善大学生心肺功能,具有可行性及现实意义。     

Exploring the impact of high intensity interval training on adolescents’ objectively measured physical activity: Findings from a randomized controlled trial

High Intensity Interval Training (HIIT) may be effective for accumulating VPA. However, the contribution of HIIT to overall physical activity is unknown. Our primary aim was to explore the impact of school-based HIIT on physical activity. The secondary aim was to explore within-individual changes in physical activity after participating in HIIT. Participants [n = 65; 15.8(0.6)years] were randomized to a HIIT or control group. Intervention groups participated in three HIIT sessions/week. GENEActiv accelerometers assessed objective physical activity at baseline and week-one, to detect changes in MPA and VPA. Intervention effects were examined using linear mixed models and evidence of a change in physical activity (i.e., compensation) were examined using multilevel linear regression models. The group-by-time interaction effects for MPA and VPA were small and moderate, respectively. Adjusted difference between groups for VPA was 1.70 min/day, 95%CI –1.96 to 5.36; p = 0.354; d = 0.55). Embedding HIIT within the school-day had a moderate effect on VPA compared to controls. Compensation analyses (i.e., individual level) suggested that adolescents were more active on days when they participated in HIIT. Further studies are needed to test the effects of HIIT on adolescents’ physical activity over extended time periods.     

Reliability and validity of Yo-Yo tests in 9- to 16-year-old football players and matched non-sports active schoolboys

The purpose of this study was to examine the test–retest reliability and construct validity of three age-adapted Yo-Yo intermittent tests in football players aged 9–16 years (n?=?70) and in age-matched non-sports active boys (n?=?72). Within 7 days, each participant performed two repetitions of an age-related intensity-adapted Yo-Yo intermittent test, i.e. the Yo-Yo intermittent recovery level 1 children's test for 9- to 11-year-olds; the Yo-Yo intermittent endurance level 1 for 12- to 13-year-olds and the Yo-Yo intermittent endurance level 2 test for 14- to 16-year-olds. Peak heart rate (HR_peak) was determined for all tests. The distance covered in the tests was 57% (1098?±?680 vs. 700?±?272?m), 119% (2325?±?778 vs. 1062?±?285?m) and 238% (1743?±?460 vs. 515?±?113?m) higher (p?≤?.016), respectively for football-trained than for non-sports active boys aged 9–11, 12–13 and 14–16 years. The typical errors of measurement for Yo-Yo distance, expressed as a percentage of the coefficient of variation (confidence interval), were 11.1% (9.0–14.7%), 10.1% (8.1–13.7%) and 8.5% (6.7–11.7%) for football players aged 9–11, 12–13 and 14–16 years, respectively, with corresponding values of 9.3% (7.4–12.8%), 10.2% (8.1–14.0%) and 8.5% (6.8–11.3%) for non-sports active boys. Intraclass correlation coefficient values for test–retest were excellent in both groups (range: 0.844–0.981). Relative HR_peak did not differ significantly between the groups in test and retest. In conclusion, Yo-Yo intermittent test performances and HR_peak are reliable for 9- to 16-year-old footballers and non-sports active boys. Additionally, performances of the three Yo-Yo tests were seemingly better for football-trained than for non-sports active boys, providing evidence of construct validity.     

The effectiveness of a high-intensity games intervention on improving indices of health in young children

This study assessed the effectiveness of a 6-week, high-intensity, games-based intervention on physiological and anthropometric indices of health, in normal weight (n = 26; 32.5 ± 8.9 kg) and obese (n = 29; 49.3 ± 8.9 kg) children (n = 32 boys, 23 girls), aged 8–10 years. Children were randomised into an exercise or control group. The exercise group participated in a twice-weekly, 40 min active games intervention, alongside their usual school physical education classes. The control group did not take part in the intervention. Before and after the intervention, participants completed both a maximal and submaximal graded exercise test. The submaximal exercise test comprised of a 6 min, moderate- and 6 min heavy-intensity bout, interspersed with a 5 min recovery. The exercise group demonstrated improvements in maximal oxygen uptake (51.4 ± 8.5 vs 54.3 ± 9.6 ml · kg^?1 · min^?1) and peak running speed (11.3 ± 1.6 vs 11.9 ± 1.6 km · h^?1), and a reduction in the oxygen cost of submaximal exercise between assessments (P < .05). A decrease in waist circumference and increase in muscle mass were observed between assessments for the obese participants randomised to the intervention (both P < .05). This study demonstrates that a short-term, high-intensity games intervention may elicit positive changes in physiological and anthropometric indices of health in normal weight and obese children.     

高强度间歇训练通过支配内脏脂肪的交感神经活动促进白色脂肪组织棕色化

目的:对比12周高强度间歇训练与中等强度持续训练促进肥胖小鼠内脏与皮下白色脂肪棕色化的效果,分析促进棕色化的交感神经活动及肌肉因子合成与棕色化标志物的相关关系。随后根据相关分析结果,对内脏脂肪交感神经受体进行化学抑制,验证交感神经活动促棕色化的作用。方法:1)效果观察实验,采用雌性C57bl/6小鼠经高糖高脂膳食诱导建立肥胖动物模型后,随机分为肥胖对照组(HFD)、中等强度持续运动组(MICT)和高强度间歇训练组(HIIT),HFD组不运动,MICT组进行中等强度跑台训练,HIIT组进行高强度间歇跑台训练。训练周期12周,期间3组均继续进行高糖高脂饲养。训练结束后测量子宫周围内脏脂肪与腹股沟皮下脂肪重量,H.E.染色观察脂肪细胞形态,蛋白免疫印迹观察骨骼肌过氧化物酶体增殖物激活受体γ辅激活因子1α(peroxisome proliferator-activated receptor gamma coactivator 1-alpha,PGC-1α)、纤维连接蛋白Ⅲ型结构域蛋白5(fibronectin typeⅢdomain-containing protein 5,FNDC5)、脂肪酪氨酸羟化酶(tyrosine hydroxylase,TH)及解偶联蛋白1(uncoupling protein-1,UCP-1)表达;2)机制验证实验,12只小鼠随机分为安慰剂组(Saline)与拮抗剂组(SR),两组均进行10天HIIT训练,SR组每次训练前2 h腹腔注射脂肪交感神经受体拮抗剂SR59230a,Saline组注射生理盐水,训练结束后蛋白免疫印迹观察内脏与皮下脂肪UCP-1表达。结果:1)HIIT组与MICT组体重均非常显著低于HFD组(P<0.01),HIIT组子宫周围内脏脂肪重量显著低于HFD组与MICT组(P<0.05),同时,内脏脂肪细胞体积HIIT组HIIT组交感神经标志物TH均高于MICT组(P<0.05或P<0.01);HIIT组内脏脂肪棕色化标志物UCP-1显著高于HFD组与MICT组(P<0.05),同时与TH呈中度相关(R=0.578,P=0.012);MICT组皮下脂肪FNCD5有升高的趋势但个体差异较大无显著性(P>0.05),内脏与皮下脂肪FNDC5与UCP-1均无显著相关性;2)SR组内脏脂肪UCP-1表达显著低于Saline组(P<0.05)。结论:HIIT相比MICT可更显著的促进内脏白色脂肪组织棕色化,且此过程与脂肪交感神经调控密切相关。