鱼虱Caligus seriolae形态结构的电镜观察

通过时寄生于石斑鱼鳃部的鱼虱的扫描电镜观察，作者对鱼虱的感觉器官、摄食器官和部分游泳足的结构进行描述．并对口器结构与摄食方式的关系、微细结构在分类中的作用、以及体表及部分附肢的附生毛的形态特点进行讨论．     

云南牛蛭部分基因注释及功能分类

以南美水蛭抗凝血Xa因子mRNA为模板设计引物,利用RT-PCR随机克隆云南牛蛭（P.yunnanensis.sp.nov）相关基因,结果得到多条片段,经严格筛选和鉴定后,确定大于200 bp ESTs序列,通过对这些ESTs序列进行聚类、拼接,结果得到10条片段,Genebank注册号分别为HQ285410,HQ285411,HQ285412,HQ285413,HQ285414,HQ285415,HQ285416,HQ285417,HQ285418,HQ285419.以上获得的云南水蛭头部和身体细胞部分转录的ESTs片段均与南美水蛭有较大差异,表明云南水蛭与南美水蛭可能使用完全不同的抗凝血机制.     

Bacillus sp.ZJU318脂肪酶发酵条件的优化及其酶学性质的研究

利用均匀设计法对Bacillus sp．ZJU318产酶的最佳培养基进行了快速优化研究。结果表明,其最适产酶培养基为：3．28％黄豆粉,0．023％蔗糖,0．075％K2HPO4,配制时的pH为12（灭菌后pH为8～9）;此外,其发酵的最佳条件为：发酵温度28℃,接种量20％,摇瓶装量20mL（250mL三角瓶）。在最适的发酵条件下,脂肪酶的发酵水平可达24．7U／mL。Bacillus sp．ZJU318脂肪酶作用的最适温度为45℃,40℃保温1h,活力几乎没有损失;但是,在50℃和60℃的条件下保温1h后,酶活降低得比较明显;酶作用的最适pH为9．0。在pH7～10,该脂肪酶较稳定,但是在酸性的环境中,酶的稳定性较低。     

桑树木腐病的初步研究

浙江省杭州的桑树和喜树感染一种层孔菌(暗黄层孔菌)后,形成树木木腐甚至枯死。发病严重度和土壤的酸碱度及湿度有密切关系。暗黄层孔菌在酸性米饭培养基培养成功。用石灰治疗有效。     

Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31

INTRODUCTION Bioflocculation is a dynamic process resulting from the synthesis of extracellular polymer by living cells. Since flocculation in microbial system was first reported by Louis Pasteur (Salehizadeh and Sho-jaosadati, 2001), bioflocculation has been investi-gated extensively and a correlation was established between the accumulation of extracellular biofloccu-lants and cell aggregation (Tenney and Verhoff, 1973). Bioflocculants are essentially polymers pro-duced by microorgan…     

8种杀菌剂对苦瓜枯萎病菌的室内毒力测定

采用生长速率测定法和分生孢子萌发抑制率法,在舍药PDA平板上和凹玻片上分别测定8种杀菌剂对苦瓜枯萎病尖孢镰刀菌的毒力作用。结果表明：20％五氯硝基苯DP对菌丝生长的抑制作用最强,EC50值为8．37mg／L,其次为40％五硝·多菌灵WP、12．5％戊唑醇WP、50％多菌灵WP和75％百菌清、聊,EC50值分别为：10．61mg／L、10．65mg／L、12．62mg／L、15．40mg／L。12．5％戊唑醇WP和对孢子萌发抑制作用最强,EC50值为3．68,20％五氯硝基苯DP、25％腈菌唑EC、40％五硝·多菌灵Ⅵ巾、50％多菌灵WP次之,EC50值分别为4．81mg／L、8．51mg／L、9．90mg／L、11．17mg／L。     

钙镁电气石检测特征探析

在宝石检测中发现，钙镁电气石呈黄绿色，与祖母绿相似。利用折射仪、红外光谱仪等手段测试确定为电气石。采用X射线衍射和电子探针分析（Cr2O含量仅0．08％），结果定名为钙镁电气石。它的主要鉴定特征与不同变种的电气石近似，与其它相似宝石的特性特征区别明显。     

Influence of medium components on elastase production using crude sources by<Emphasis Type="Italic">Bacillus</Emphasis> sp. EL31410

A newly isolated strain EL31410, producing elastase (E. C3. 4. 4. 7) with high elastolytic activity was identified asBacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production. A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4% (w/v) wheat bran and 9.4% (v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase production in the optimized medium composition was also described. Project (No. 300024) supported by the Zhejiang Provincial Natural Science Foundation of China     

营养和寄生性膜翅目的性分化 3.移植平腹小蜂卵后的性比变化

寄主卵大小及新鲜陈旧会影响平腹小蜂的性比本试验用移植卵的方法，证实了影响该蜂性比的关键在于：靠平腹小蜂产卵瓣上的感觉器感知寄主卵的大小及新鲜程度，通过神经肌肉控制受精囊是否释放精子入卵，决定产下的是受精卵或非受精卵，因而影响子代蜂的性比．     

小球藻生物吸附废水中铜的研究

以小球藻作为生物吸附剂,去除水溶液中微量Cu~(2+),研究结果表明小球藻对Cu~(2+)的生物吸附主要经历了快速的吸附和缓慢的吸收两个步骤:pH值是影响Cu~(2+)生物吸附的一个重要影响因素,最佳pH值在6～8之间;离子强度对Cu~(2+)的吸附有一定的抑制作用;小球藻对Cu~(2+)的生物吸附符合Freundlich等温方程。