| ELISA法检测三种类型的类风湿因子 |
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| 引用本文： | 史立,甘晓丹,董怡. ELISA法检测三种类型的类风湿因子[J]. 河北北方学院学报(医学版), 1994, 0(3) |
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| 作者姓名： | 史立 甘晓丹 董怡 |
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| 作者单位： | 北京协和医院内科(史立，甘晓丹)，北京协和医院内科(董怡) |
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| 摘 要： | 用ELISA法建立了检测IgM、IgG、IgA三种类风湿因子。在类风湿关节炎(RA)中IgM-RF,IgG-RF,IgA-RF的阳性率分别为67.1％,58.6％,42.8％,明显高于其他结缔组织病,且OD值也高。ELISA法检测IgM-RF的阳性率明显高于乳胶凝集法检测的阳性率,但特异性一样为2.3％,但弥补了乳胶凝集法只能检测IgM-RF的不足,尤其是建立了IgG-RF和IgA-RF的检测,对临床诊断有很大帮助,我们还发现IgA-RF和SS有一定关系。
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| 关 键 词： | 酶联免疫吸附法 类风湿因子 类风湿关节炎 |
ELISA METHOD OR DETERMINATION OF IgM-, IgG-, IgA-RHEUMATOID FACTOR |
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| Shi Li. ELISA METHOD OR DETERMINATION OF IgM-, IgG-, IgA-RHEUMATOID FACTOR[J]. Journal of Hebei North University:Medical Edition, 1994, 0(3) |
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| Authors: | Shi Li |
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| Abstract: | We set up a ELISA method for the determination of IgM-, IgG-,IgA-type of rheumatoid factor (RF) and report the result in patients with rheumatoid arthritis (RA), systemic lupus erthematosus (SLE), Sjogren syndrome (SS) and other connective tissue diseases.It was found that the positive rates of IgM-RF 67.1%, IgG-RF 58.6%, IgA-RF42.8%, as well as the OD value in patients with RA were higher than that i n patients with other connective tissue diseases.Our results also revealed the specifcity of ELISA for RF was 2.3%,same as the Latex aggutiniation method. Furthermore.there seemed to be a profound correlation of IgA-RF with SS patients. |
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| Keywords: | ELISA rheumatoid factor rheumatoid arthritis |
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