霍山石斛工厂化繁育技术研究

以野生霍山石斛种子为外植体,对霍山石斛组培快繁技术进行研究,旨在为霍山石斛规模化、标准化、产业化生产提供依据。结果表明,种子萌发与原球茎诱导最适培养基为MS+6-BA0.1mg/L+NAA0.5mg/L+20%土豆汁;原球茎继代增殖与分化最适培养基为1/2MS+6-BA0.5mg/L+NAA0.1mg/L+20%土豆汁;丛生芽增殖最适培养基为1/2MS+6-BA0.5mg/L+NAA0.5 mg/L+20%土豆汁;壮苗生根最适培养基为1/2MS+NAA0.5 mg/L+20%土豆汁;炼苗最适温度、光照及时间:温度(24±2)℃,光照6000Lx,炼苗时间10d,后3d将瓶盖打开;试管苗移栽最适基质配方:草碳根+碎木屑+碎石(1∶1∶1)。

Research on Dendrobium Huoshanense Factory Breeding

In order to provide the basis for the scale,and standardization,industrialization production of Dendrobium huoshanense,research was taken on tissue rapid propagation of Dendrobium huoshanense,which uses the wild Dendrobium huoshanense seed as explants.The results showed that the most suitable medium for seed germination and protocorm induction was MS +6-BA 0.1 mg / L + NAA0.5 mg / L +20% potato juice;the most suitable medium for protocorms continued metabolic reproduction and differentiation was 1/2MS+6-BA 0.5 mg / L + NAA 0.1 mg / L,+20% potato juice;the most suitable medium for buds increase and multiply was 1/2MS +6-BA 0.5 mg / L + NAA0.5 mg / L +20% potato juice;the most suitable medium for strong seedling rooting was 1/2MS + NAA0.5 mg / L +20% potato juice;the optimum temperature,light and time for seedling acclimatization is at temperature of(24±2) ℃,light: 6000Lx,time:10days and open the cap during the last 3 days;the most suitable formula for plantlets transplant was grass carbon root + wood chips+ Gravel(1∶ 1∶ 1).