NIDDM合并肺部感染患者的免疫功能变化研究

[目的]通过对免疫球蛋白(IgA、IgG、IgM),补体(C3、C4),T淋巴细胞亚群(CD3、CD4、CD8),可溶性白细胞介素2受体(SIL-2R)的检测,研究这些免疫指标与NIDDM(非胰岛素依赖性糖尿病)合并肺部感染患者的关系.[方法]采用双抗夹心法测SIL-2R,速率散射比浊法测免疫球蛋白和补体,单克隆法测淋巴细胞亚群等免疫指标.[结果]①糖尿病及其合并感染组SIL-2R显著高于正常对照组(P<0.01).CD4/CD8显著低于正常对照组(P<0.05);②合并感染组较正常对照组IgG、IgA、C3、C4显著升高(P<0.05、P<0.01、P<0.05、P<0.05),IgM无显著变化(P<0.05).[结论]NIDDM合并肺部感染可加重糖尿病及机体免疫功能低下,NIDDM合并肺部感染T淋巴细胞亚群(CD3、CD4、CD8),补体(C3、C4)免疫球蛋白(IgA、IgG)可溶性白细胞介素2受体(SIL-2R)的检测,可作为糖尿病及其合并感染者的病情变化的客观指标.

Disorder of immune function in type Ⅱ diabetics complicated with pulmonary infection

[Objective] To study the relation of Immunoglobulins, (IgA, IgG, IgM), complements (C3, C4), T-lymphocyte Subsets (CD3, CD4, CD8), serum soluble interleukin-2 receptor(SIL-2R) in diabetics complicated with pulmonary infection. [Methods] The level of serum soluble interleukin - 2 receptor(SIL - 2R) was detected by the ELISA. Immunoglobulin and Complement were measured by the nephellometry. The percent of T-lymphocyte Subsets was observed by Monodon. [Results] (1) The level of SIL-2R in diabetic patients with pulmonary infection was significantly higher that in normal control group (P<0.01). CD4/CD8 in diabetic patients with infection was lower significantly lower than that in normal control group (P<0.05) than (2) IgG, IgA, C3 and C4 in diabetic patients with pulmonary infection was significantly higher than that in normal control groups (P<0.05, P<0.01, P<0.05, P<0.05). [Conclusion]Pulmonary infection may depress the host immunity in diabetic patients. The dynamic estimation of T-lymphocyte Subsets (CD3, CD4, CD8), complement (C3, C4), Immunoglobulin (IgA, IgG) and serum soluble interleukin-2 receptor (SIL-2R) levels would be taken as indicators of NIDDM complicated infection.