Promoter trapping in Magnaporthe grisea |
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Authors: | Xiao-hong Liu Jian-ping Lu Jiao-yu Wang Hang Min Fu-cheng Lin |
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Institution: | (1) Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China;(2) School of Life Sciences, Zhejiang University, Hangzhou, 310029, China |
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Abstract: | Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures ofM. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis. |
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Keywords: | Promoter trapping Green fluorescent protein Magnaporthe grisea |
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