| 紫外诱变酿酒酵母筛选耐高温菌株 |
| |
| 引用本文： | 李艾,李云凯,程磊.紫外诱变酿酒酵母筛选耐高温菌株[J].唐山学院学报,2013,26(3):35-37. |
| |
| 作者姓名： | 李艾 李云凯 程磊 |
| |
| 作者单位： | 唐山学院环境与化学工程系,河北唐山,063000 |
| |
| 摘 要： | 以实验室保存的酿酒酵母为初始菌株，采用紫外分光光度法测定菌株浓度，绘制出菌株生长曲线，由此确定出最佳诱变菌龄为3～10h，在此期间利用紫外线对菌株进行诱变，筛选出一株适于在高温下发酵的菌株，紫外线诱变条件为：紫外灯功率10w，照射距离30cm，照射时间3min。此菌株在37℃下培养72h，其成熟发酵醪酒精产率为3．837％。
|
| 关 键 词： | 酿酒酵母 菌株 诱变 耐高温 |
Screening of Saccharomyces Cerevisiae with Temperature Tolerance Radiated by UV |
| |
| LI Ai
,
LI Yun-kai
,
CHENG Lei.Screening of Saccharomyces Cerevisiae with Temperature Tolerance Radiated by UV[J].Journal of Tangshan College,2013,26(3):35-37. |
| |
| Authors: | LI Ai LI Yun-kai CHENG Lei |
| |
| Institution: | LI Ai,LI Yun-kai,OHENG Le (Environmental and Chemical Engineering, Tangshan College, Tangshan 063000, China) |
| |
| Abstract: | In this study, saccharomyces cerevisiae preservation in laboratory is activated and puri- fied. The growth characteristics of saccharomyces cerevisiae is analyzed in the study and its growth curve is drawn by UV spectrophotometry. The best mutational age of strain is 3-10 h in which saccharomyces cerevisiae is radiated by UV. One saccharomyces cerevisiae strains which can be adapted to high temperature fermentation is screened out, UV mutagenesis conditions are as follows： mutagenic time,3min~the mutagenic distance,30 cm; UV lamp power 10 W. The bacte- rial strain is cultured 72 h at 37 ℃ measured mature fermentation alcohol production rate of 3.837%. |
| |
| Keywords: | saccharomyces cerevisiae bacterial strain mutagenesis temperature tolerance |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
