Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability |
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Authors: | Zhang-yue Song Jing-luan Tian Wei-zhe Fu Lin Li Ling-hong Lu Lian Zhou Zhi-hui Shan Gui-xiang Tang Hui-xia Shou |
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Institution: | 13400. College of Life Sciences, Zhejiang University, Hangzhou, 310058, China 23400. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China 33400. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China
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Abstract: | The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation. Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system. In this study, twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection, regeneration capacity, and stable transgenic efficiency. Three genotypes, Yuechun 04-5, Yuechun 03-3, and Tianlong 1, showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system. For the Tianlong 1, the average stable transformation efficiency is 4.59%, higher than that of control genotypes (Jack and Williams 82), which is enough for further genomic research and genetic engineering. While polymerase chain reaction (PCR), LibertyLink strips, and β-glucuronidase (GUS) staining assays were used to detect the insertion and expression of the transgene, leaves painted with 135 mg/L Basta could efficiently identify the transformants. |
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