Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn,wheat, and feed samples |
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| Authors: | Xian Zhang Ke He Yun Fang Tong Cao Narayan Paudyal Xiao-feng Zhang Hou-hui Song Xiao-liang Li Wei-huan Fang |
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| Institution: | 1.China-Australian Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology,Zhejiang A&F University,Lin’an,China;2.Zhejiang University Institute of Preventive Veterinary Medicine,Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine,Hangzhou,China;3.Zhejiang Academy of Science and Technology for Inspection and Quarantine,Hangzhou,China |
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| Abstract: | A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53?12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06?39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control. |
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