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Development of a pyrF-based counterselectable system for targeted gene deletion in Streptomyces rimosus |
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| Authors: | Yang Yiying Sun Qingqing Liu Yang Yin Hanzhi Yang Wenping Wang Yang Liu Ying Li Yuxian Pang Shen Liu Wenxi Zhang Qian Yuan Fang Qiu Shiwen Li Jiong Wang Xuefeng Fan Keqiang Wang Weishan Li Zilong Yin Shouliang |
| Institution: | 1.School of Life Sciences, North China University of Science and Technology, Tangshan, 063210, China ;2.State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China ;3.Shengxue Dacheng Pharmaceutical Co., Ltd., Shijiazhuang, 051430, China ; |
| Abstract: | Journal of Zhejiang University-SCIENCE B - Streptomyces produces many valuable and important biomolecules with clinical and pharmaceutical applications. The development of simple and highly... |
| Keywords: | Counterselectable system pyrF 5-Fluoroorotic acid (5-FOA) Gene deletion Streptomyces rimosus |
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