Bio-electrospraying and aerodynamically assisted bio-jetting whole human blood: Interrogating cell surface marker integrity |
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| Authors: | Pascal Joly Naina Chavda Ayad Eddaoudi and Suwan N Jayasinghe |
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| Institution: | 1.BioPhysics Group, Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom;2.Flow Cytometry Unit, Haematology Department, University College London Hospitals, 60 Whitfield Street, London W1T 4EU, United Kingdom;3.Flow Cytometry Core Facility, Camelia Botnar Laboratories, Institute of Child Health/Great Ormond Street Hospital for Children, University College London, 30 Guilford Street, London WC1N 1EH, United Kingdom |
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| Abstract: | Bio-electrospraying and aerodynamically assisted bio-jetting are two direct cell handling approaches recently pioneered, which have demonstrated significant applicability to the life sciences. These two bioprotocols have undergone scientific rigor, which have seen these techniques been explored in conjunction with a wide range of immortalized, primary and stem cells, and those whole organisms. Those studies have demonstrated a cellular population of >70% viable post-treatment in comparison with controls. Although, these studies assessed cellular viability, cell surface molecules play a critical role in several cellular functions, in particular, have importance to tissue engineering and regenerative medicine. Thus, in the studies reported herein, we demonstrate post-treated viable cells retain their cell surface marker expression levels in comparison to controls, over both short and long time points. Therefore, these studies further push back the frontiers of both bio-electrosprays and aerodynamically assisted bio-jetting in their endeavor as novel strategies for tissue engineering and regenerative biology∕medicine with possible targeted clinical utility. |
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