Estimation of the levels of atrial natriuretic peptide (ANP) in cardiac blood samples by a simple and indigenous enzyme linked immunosorbent assay

Levels of Atrial Natriuretic Peptide (ANP) were estimated in twenty four blood samples collected from the right and the left ventricles of the patients undergoing cardiac catheterization by an Enzyme Linked Immunosorbent Assay (ELISA) developed to the sensitivity of 5 pp/well and within 10% of interassay coeficient of variance. Simultaneously, levels of ANP in plasma samples from the systemic venous blood of seventy five normal subjects were also estimated which ranged between 25–60 pg/ml.