三种三尖杉属植物越冬期光合器的功能和膜蛋白成分的比较研究

本文对三尖杉属中三种植物即：三尖杉Cephalotaxus fortunei，粗榧C.sinensis 和柱   冠日本粗榧C.harringtonia cv．Fastigiata越冬期的光合器功能进行了研究。研究结果表明   这三种常绿三尖杉植物越冬时叶绿体的光合放氧活性受到抑制，但光系统II中心的活性和镁   离子调节光能分配的功能存在。说明其越冬特性与松柏科植物类似。     叶绿体膜的低温荧光测定结果表明三种植物的F685,F695和F735的比率各不相同。用SDS—   聚丙烯酰胺凝胶电泳分析叶绿体膜蛋白，发现柱冠日本粗榧的多肽数与三尖杉和粗榧有明显差异。以上特征可能有助于分类或系统位置的研究。

A Comparative Study on Function and Membrane Proteins of Chloroplasts of Three Species of Cephalotaxus in Late Winter

Three species of evergreen Cephalotaxus (C. fortunei Hook. f., C. sinensis Li and C. harringtonia cv. Fastigiata) were used as materials to study some functional   properties of chloroplasts.  It is found that the oxygenevolving capacity of the chloroplasts from these plants is inhibited but partial reaction of PS-II and effect of Mg2+ on energy distribution between two photosystems are detectable during the winter. Seasonal effects on the functional properties of chloroplasts from evergreen Cephalotaxus are similar to that of conifer chloroplasts.       The ratio between F685, F695 and F735 of fluorescence emission spectra at 77°K of ch- loroplasts is different among these three species. It is found by using SDS-PAGE that the num- ber of polypeptide resolved from thylakoid membrane of C. harringtonia cv. Fastigiata sub- stantially differs from that of C. fortunei Hook.f. and C. sinensis Li. The result shows that the fluorescence emission spectrum feature and polypeptide composition of thylakoid membrane may be used as a tool for systematics of the genus Cephalotaxus.