| 福建大豆主栽品种的组织培养研究 |
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| 引用本文： | 张戊英,张韵春,张蔚勇.福建大豆主栽品种的组织培养研究[J].闽西职业技术学院学报,2006,8(1):117-119. |
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| 作者姓名： | 张戊英 张韵春 张蔚勇 |
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| 作者单位： | 1. 闽西职业技术学院,资源工程系,福建,龙岩,364021
2. 前埔中学,福建,厦门,361000 |
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| 摘 要： | 利用福建大豆主栽品种的子叶节为外植体,进行组织培养再生研究,探讨不同品种、不同激素和除草剂不同浓度的诱导效果。结果表明不同大豆品种诱导分化能力差别较大,供试品种中“莆豆8008”的诱导能力较强,丛生芽诱导率和平均丛生芽数分别为94.2%和2.69。不同激素种类和浓度对大豆子叶节丛生芽诱导,表明6-BA较适合于丛生芽的诱导,适宜浓度为1.67mg/L。大豆子叶节不定芽分化和伸长的除草剂Glufosinate梯度实验表明除草剂Glufosinate5mg/L可作为不定芽分化和伸长的选择压力,生根培养基上不宜加除草剂Glufosinate。
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| 关 键 词： | 大豆 子叶节 组织培养 激素 Glufosinate |
| 文章编号： | 1673-4823(2006)01-0117-03 |
| 收稿时间： | 11 11 2005 12:00AM |
| 修稿时间： | 2005年11月11 |
Tissue culture of Fujian soybean cultivar |
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| ZHANG Wu-Ying,ZHANG Yun-Chun,ZHANG Wei-Yong.Tissue culture of Fujian soybean cultivar[J].Journal of Minxi Vocational and Technival College,2006,8(1):117-119. |
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| Authors: | ZHANG Wu-Ying ZHANG Yun-Chun ZHANG Wei-Yong |
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| Abstract: | The regeneration of cotyledon node explants was studied in several varities of soybean(Glycine.Max.L.).Among soybean cultivar suppled,pudou number 8 has comparatively frequency of mltiple shoots initiation with 94.1%.The regeneration of cotyledon node explants was studied in pudou number 8 with different plant hormane and different concentration.The result indicates 6-BA1.67mg/L is the best.Soybean cotylendon node was initiated on shoot initiation medium and shoot elongation medium with different concentration of glufonsinate.The result indicates glufonsinate at 5 mg/L concentration suppressed the shoot initiation and shoot elongation. |
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| Keywords: | soybean(Glycine max L ) cotyledon node tissue culture plant hormane Glufosinate |
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