首页 | 本学科首页   官方微博 | 高级检索  
     检索      

驱蚊香草组织培养与快速繁殖
引用本文:林亮亮.驱蚊香草组织培养与快速繁殖[J].宁德师专学报(自然科学版),2008,20(1):18-21.
作者姓名:林亮亮
作者单位:福建农业职业技术学院现代教育中心,福建福清350119
摘    要:以驱蚊香草不同部位为外植体,进行离体快繁试验,结果表明:外植体以叶片和顶芽容易诱导产生愈伤组织,最高诱导率达90%;诱导培养基以MS+6-BA1.0—2.0mg·L^-1+NAA0.2mg·L^-1效果较好,平均芽诱导分化率达85.6%一86.7%;增殖培养基以MS+6-BA1.5mg·L^-1+NAA0.1mg·L^-1效果最好,丛生芽增殖量多,繁殖系数达6倍以上;用MS+6-BA1.5mg·L^-1+NAA0.1mg·L^-1培养基进行壮苗培养,诱导生根培养基以1/2MS+NAA0.1—0.3mg·L^-1,生根率达到100%;假植20d后,移栽成活率达100%,本结果为驱蚊香草离体无性繁殖系的建立提供参考.

关 键 词:驱蚊香草  离体快繁  培养基

Tissue culture & fast regeneration of cymbopogon citrates
LIN Liang-liang.Tissue culture & fast regeneration of cymbopogon citrates[J].Journal of Ningde Teachers College(Natural Science),2008,20(1):18-21.
Authors:LIN Liang-liang
Institution:LIN Liang - liang (Modem Education Center, Fujian Agricultural Vocation- technical College ,Fuqing Fujian 350119, China)
Abstract:This experiment utilizes various regeneration, whose result demonstrates that callus by inducement, and the maximum rate MS+6-BA1.0-2.0mg·L^-1 +NAA0.2 differentiation rate reaching 85.6 -86.7% ;the proliferation culture medium of MS + 6 - BA 1.5 mg ·L^-1 + NAA0.1 mg ·L^-1 highlights the best result, with the largest number of clustering buds, the reproduction quotiety is 6 times. I use culture medium MS +6 - BA 1.5mg·L^-1 + NAA0.1 mg ·L^-1 to enable strong seedlings, while using 1/2MS + NAA0. 1 -0.3rag ·L^-1 as radication inducement culture medium, with 100% of radication rate; the survival ratio of the transplanted seedlings reaches 100% after 20 days of temporary planting. This experiment result provides references for the formation of cymbopogon citrates explanting vegetative propagation.
Keywords:cymbopogon ctrates  apart fast regeneration  culture medium
本文献已被 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号