溶胶-凝胶杂化膜固定介体和酶的过氧化氢、葡萄糖生物传感器

以通过溶胶-凝胶技术制备的SiO2/Nafion杂化膜固定辣根过氧化物酶,以杂化膜中的Nafion固定的亚甲基蓝为辣根过氧化物酶和玻碳电极间的电子传递介体,制成了电流型单酶过氧化氢生物传感器。在此基础上通过固定双酶（辣根过氧化物酶-葡萄糖氧化酶）制成了葡萄糖生物传感器。探讨了杂化膜的制备条件、生物传感器的性能和工作电位、pH值、温度、干扰物质等对生物传感器的影响。单酶生物传感器线性响应范围为1.0×10-6～1.6×10-4mol/L,检测限为6.0×10-7mol/L（S/N=3）,达到95%稳态响应电流用时少于15s。双酶葡萄糖生物传感器线性响应范围为7.8×10-6～2.4×10-3mol/L。检测限为4.2×10-6mol/L（S/N=3）,达到95%稳态响应电流用时少于25s。

Amperometric Biosensor for Hydrogen Peroxide and Glucose Based on Methylene Blue and Enzyme Incorporated into SiO_2/Nafion hybrid Film

An amperometric biosensor highly sensitive to H2O2 is constructed by immobilizing horseradish peroxidase(HRP) and Methylene Blue into the sol-gel-derived SiO2/Nafion hybrid film.The presence of Nafion in the sol-gel-derived hybrid film not only prevents the cracking of the pure sol-gel-derived SiO2 film but also immobilizes positively charged organic dyes such as methylene blue(MB).The immobilized MB acts as electron transfer mediator between horseradish peroxidase and glass carbon electrode.Similarly,an am...