麻鸭血替代人血测定HBsAg-ELISA法在校内实训中的应用研究

为了避免实验室生物安全事故（学生感染和实验室污染）的发生,探讨采用麻鸭血替代人血用酶联免疫吸附试验（ELISA法）作乙肝病毒表面抗原（HBsAg）检测的可行性。方法：收集黔东南地区100份麻鸭血标本,采用ELISA法检测HBsAg。结果 100份麻鸭血清标本HBsAg检测结果均为阴性。结论：我国多地报道麻鸭血携带鸭乙肝病毒（DHBV）,但通过检测,我地区麻鸭血HBsAg为阴性,在校内实训课中可作为标本应用。

The Research of The Testing Way of HbsAg - ELISA with Ma Duck Blood Replacing Human Blood in the training Courses in Our College

purpose In order to avoid the accidents （infection on the students and pollution to the lab） in the biological lab, we have explored the possibility of testing Hepatitis B virsu Surface Anti C, en（HbsAg） in the way of Enzyme Linked Immunosorbent Assay （ ELISA）, with the Ma Duck blood replacing the human blood. Methodology Collect the blood from 100 Ma ducks in the area of Qiandongnan as the samples, then test HBsAg using the way of ELISA. Results All the blood samples from the 100 Ma ducks show the test resuls are negative. Conclusion As reported, Ma duck blood carries the Duck Hepatitis B Virus （DHBV） in most areas of China, however, Ya duck blood in the area of Qiandongnan is negative in the test, so it can be appiled for samples in the training courses.