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多因素对生姜蛋白酶活性的影响
引用本文:郝桂霞,杨秋菊. 多因素对生姜蛋白酶活性的影响[J]. 韩山师范学院学报, 2005, 26(3): 71-75
作者姓名:郝桂霞  杨秋菊
作者单位:韩山师范学院化学系,广州,潮州,521041
摘    要:实验采用以酪蛋白为底物的茚三酮显色法,综合研究了pH值、温度、激活SJ(EDTA)、金属离子(K^+、Mg^2+)、以及贮存时间和贮存温度对生姜蛋白酶活性的影响,结果表明:生姜蛋白酶耐高温,稳定性好,能耐受一定的pH值和温度变化.在各个影响因素中,温度对生姜蛋白酶的影响最大,而金属离子K^+的影响最小.

关 键 词:生姜蛋白酶 酶活性 茚三酮显色法
文章编号:1007-6883(2005)03-0071-05
收稿时间:2004-01-14
修稿时间:2004-01-142004-09-14

The Influence of Factors on the Activity of Ginger Rhizome Protease
HAO Gui-xia,YANG Qiu-ju. The Influence of Factors on the Activity of Ginger Rhizome Protease[J]. Journal of Hanshan Teachers College, 2005, 26(3): 71-75
Authors:HAO Gui-xia  YANG Qiu-ju
Affiliation:Department of Chemistry, Hanshan Teachers College, Chaozhou 521041, China
Abstract:This article discusses the effect of several factor on ginger rhizome protease activities by the way of ninidrin development process,which include pH, temperature,activators(EDTA), positive tons(K+,Mg2+),and storing time and storing temperature. The result show that ginger rhizome protease can endure a certain high temperature, has a stabilization, Also, it can endure a certain rang of pH and temperature. Among the factors, the most effection factor is temperature, and the least is the consistency of K+.
Keywords:ginger Rhizome protease   proteolytic activity   ninidrin development process
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