金莲花中荭草苷和牡荆苷对D-半乳糖致衰老小鼠脑组织动态抗氧化作用研究

目的：研究金莲花中荭草苷和牡荆苷对D-半乳糖（D-galactose，D-Gal）致衰老小鼠脑组织抗氧化酶活性的动态影响，探讨荭草苷和牡荆苷对脑组织抗氧化酶系活性作用的时效关系。方法：采用D-Gal腹腔注射（intraperitoneal injection，ip）致亚急性衰老小鼠模型，8周造模成功后，分成正常组、模型组、荭草苷和牡荆苷各三个剂量组（40，20，10 mg·kg-1），维生素E（20 mg·kg-1）对照组。各治疗组从第9周开始每日上午分别灌胃（intragastric perfusion，ig）给药，每日一次。给药第2，4，6，8周时，分别断头处死小鼠，用预冷的生理盐水制成10％的脑组织匀浆，8000 rpm离心得上清液，检测超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶以及脑脂褐素含量。结果：与模型组相比，给药2，4周后，各治疗组抗氧化酶系虽有提高但差异无统计学意义，给药6，8周后，各治疗组在提高抗氧化酶系活性以及降低脑脂褐素含量上差异有显著性。荭草苷组和牡荆苷组相比，给药2，4周后，各剂量组在提高抗氧化酶活性及降低脑脂褐素含量上差异无统计学意义，给药6周后，荭草苷40，20，10 mg·kg-1三个剂量组在提高抗氧化酶活性及降低脑脂褐素含量上优于同等剂量的牡荆苷，给药8周后，荭草苷20 mg·kg-1和10 mg·kg-1剂量组在提高抗氧化酶系的活性及降低脑脂褐素含量上高于同等剂量的牡荆苷，而40 mg·kg-1剂量组差异无显著性。与维生素E组相比，给药8周后，荭草苷和牡荆苷20，10 mg·kg-1两个剂量组在提高脑组织抗氧化酶系活性及降低脑脂褐素含量上低于维生素E组，40 mg·kg-1剂量组差异无显著性。结论：荭草苷和牡荆苷具有提高D-Gal致衰老小鼠脑组织抗氧化酶活性的作用，随给药时间和给药剂量的增加，其提高抗氧化酶活性的作用增强。40 mg·kg-1的荭草苷和牡荆苷抗氧化作用?

Study on Dynamical-antioxidation of Orientin and Vitexin in Trollius Chinensis to Brain Tissue in D-galactose-induced Aging Mice

Objective：To study antioxidant enzyme activity and lipofuscin dynamic effects of orientin and vitexin in Trollius chinensis on aging mice induced by D-galactose（D-Gal）and find the time effect relationship of antioxidant. Methods：The aging mice model was induced by intraperitoneal injection of D-Gal,and it was successful after 8 weeks. Divided into blank group,model group,high,medium and low dose group of orientin and vitexin,Vitamin E control group. From the 9 th week,every morning each group will be given medicine. The route was＆amp;nbsp;oral administration,and the time was 8 weeks. Decapitated mice at the 2 th ,4 th ,6 th and 8 th week,resected brain in frozen conditions,10% tissue homogenate was made with NS solution, then got supernatant by use of centrifugation in 8 000 rpm,detected superoxide dismutase（SOD）, catalase（CAT）,glutathione peroxidase（GSH-PX）and lipofuscin（LPF）. Results：Compared with model group,the activity of antioxidase in each therapy group increased slowly after 2 and 4 weeks,but not statistically significant,and after 6 and 8 weeks,orientin and vitexin significantly improved the activity of antioxidase,and significantly reduced the amount of LPF. Compared orientin with vitexin,the activity of antioxidase and the amount of LPF in the same dose group was no statistically significant after 2 and 4 weeks. The activity of antioxidase in each dose group of orientin was better than that of the vitexin of the same dose. They significantly reduced lipofuscin levels in the brain after 6 weeks and orientin was superior to vitexin at the same dose. The activity of antioxidase in medium and low dose group of orientin was better than the same vitexin,they significantly reduced lipofuscin levels in the brain and high dose group have no significant difference after 8 weeks.Compared with VE group,after 8 weeks,the activity of antioxidase in medium and low dose group of orientin and vitexin were lower than VE group, they have significant difference,meanwhile,high dose group have no