| 凝胶过滤及离子交换层折法纯化玉米CuZn—SOD的研究 |
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| 引用本文： | 文才艺,张艳红.凝胶过滤及离子交换层折法纯化玉米CuZn—SOD的研究[J].襄樊学院学报,2001,22(2):88-92. |
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| 作者姓名： | 文才艺 张艳红 |
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| 作者单位： | 1. 襄樊学院,化学化工系,湖北,襄樊,441053
2. 华东理工大学,生物工程学院,上海,200237 |
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| 摘 要： | 用葡聚糖（Sephadex G－75）凝胶过滤及纤维素（DEAE－32）离子交换层析法分离纯化玉米CuZn－SOD,并对其部分理化性质进行了测定。结果表明，玉米CuZn－SOD具有怀其它来源的CuZn－SOD相似的性质，即最大吸收峰为260nm，分子量为33，600道尔顿，分子中含有17种289个氨基酸残基，不含色氨酸，N－末端氨基酸为丙氨酸，1分子酶中含2个铜原子和2个锌原子；电泳分析显示，蛋白质谱带和酶活性谱带数目相同，均为两条。
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| 关 键 词： | 玉米超氧化物歧化酶 葡聚糖凝胶过滤 纤维素主子交换层析 分离 纯化 氨基教 蛋白质 酶活性 |
| 文章编号： | 1009-2854(2001)02-0088-05 |
| 修稿时间： | 2001年1月20日 |
Study on Seperation and Purification CuZn-SOD from Maize by the Methods of
Sephadex G-75 Chromatography and DEAE-32 Ion-exchange Chromatography |
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| WEN Cai-yi,ZHANG Yan-hong.Study on Seperation and Purification CuZn-SOD from Maize by the Methods of
Sephadex G-75 Chromatography and DEAE-32 Ion-exchange Chromatography[J].Journal of Xiangfan University,2001,22(2):88-92. |
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| Authors: | WEN Cai-yi ZHANG Yan-hong |
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| Institution: | WEN Cai-yi1,ZHANG Yan-hong2 |
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| Abstract: | Seperation and purification CuZn-SOD from maize by the methods of Sephadex G-75 chromatography and DEAE-32 ion-exchange chromatography and determination of CuZn-SOD qualities have been done .Results show that CuZn-SOD from the maize has the same characterization as the other one from different sources,and the main characterizations are as follow: the 260nm biggest ultraviolet absorption , 33600 Dalton molecular weight ,with there are seventeen kinds and two hundred eighty-nine amino acid residues, no Tryptophan in the molecular, and N-terminal amino acid is Alanine as well as two copper and two zine in one SOD molecular. There are two protein bands and two enzyme bands in the PAGE pattern. |
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| Keywords: | Superoxide dismutase in maize Sephadex G-75 chromatography DEAE-32 ion-exchange chromatography Seperation Purification |
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