| 大花蕙兰组培快繁技术的研究 |
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| 引用本文： | 杨进,彭春雷.大花蕙兰组培快繁技术的研究[J].荆门职业技术学院学报,2005,20(3):17-21. |
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| 作者姓名： | 杨进 彭春雷 |
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| 作者单位： | 荆门职业技术学院生物工程系,湖北荆门448000 |
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| 基金项目： | 荆门职业技术学院科学研究计划项目,项目编号2002005. |
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| 摘 要： | 以大花蕙兰的茎尖为外植体，探讨了不同培养基对原球茎增殖、分化和小苗诱导及壮苗生根的影响．结果表明：1)大花蕙兰组织培养的原球茎诱导和增殖都可以用培养基1／2MS BA1．0mg／L NAA0．5mg／L；2)适合大花蕙兰组织培养原球茎芽苗诱导的培养基配方为1／2MS BAl．5mg／L NAA0．5mg／L；3)适合大花蕙兰壮苗生根的培养基配方为1／2MS NAA0，1mg／L GAl．0mg／L的组合．
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| 关 键 词： | 大花蕙兰 组培快繁技术 培养基配方 组织培养 壮苗生根 原球茎增殖 外植体 诱导 茎尖 小苗 芽苗 |
| 文章编号： | 1008-4657(2005)03-0017-05 |
| 修稿时间： | 2004年12月16 |
Study on the Technical System of Tissue Culture and Rapid Propagation in Cymbidium Grandiflorium |
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| YANG Jin,PENG Chun-lei.Study on the Technical System of Tissue Culture and Rapid Propagation in Cymbidium Grandiflorium[J].Journal of Jingmen Vocational Technical College,2005,20(3):17-21. |
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| Authors: | YANG Jin PENG Chun-lei |
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| Abstract: | The effects of inducement of protocorm-like bodies (PLB) in the different medium with stem apex as explants,and of multiplication and differentiation of PLB in different medium with PLB as explants were compared. The results were as follows:1) The best medium for the inducement and multiplication of PLB both were 1/2MS+BA 1.0mg/L+NAA 0.5mg/L,so it could reduce a medium preparation; 2) The best medium for the developing of shoots from PLB was 1/2MS+BA 1.5mg/L+NAA 0.5mg/L;3)The best medium for the developing of roots from PLB was 1/2MS+NAA 0.1mg/L+GA1.0mg/L. |
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| Keywords: | Cymbidium grandiflorium protocorm-like bodies (PLB) tissue culture seedling multiplication radication |
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