| 云南松松塔中原儿茶酸提取工艺的研究 |
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| 引用本文： | 吴道勋,;刘璐,;王莹,;刘熙,;张海珠.云南松松塔中原儿茶酸提取工艺的研究[J].大理师专学报,2014(4):4-7. |
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| 作者姓名： | 吴道勋 ;刘璐 ;王莹 ;刘熙 ;张海珠 |
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| 作者单位： | [1]大理学院药学与化学学院,云南大理671000; [2]云南省昆虫生物医学研发重点实验室,云南大理671000 |
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| 基金项目： | 国家自然科学基金资助项目（81260632） |
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| 摘 要： | 目的：优化云南松松塔中原儿茶酸的提取工艺。方法：以不同溶剂对云南松松塔中原儿茶酸的提取效率为指标,用高效液相色谱法进行含量测定,采用L9（3^4）正交设计试验以确定最佳提取工艺。结果：最佳提取工艺：以乙酸乙酯为提取溶剂,加入0.1 mol/L的HCl 3 mL,料液比为1∶40（mg/mL）,超声时间90 min,超声温度40℃。结论：优化后的提取工艺简单、稳定、提取率高。
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| 关 键 词： | 云南松 松塔 原儿茶酸 正交试验 |
Extraction Process of Protocatechuic Acid in the Pinecone of Pinus yunnanensis |
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| Institution: | WU Daoxun,LIU Lu,WANG Ying,LIU Xi, ZHANG Haizhu ( 1 .College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2.Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R＆D, Dali, Yunnan 671000, China) |
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| Abstract: | Objective：To optimize the extraction process of the pinecone of protocatechuic acid in Pinus yunnanensis. Methods： Different solvents were used to extract protocatechuic acid in Pinus yunnanensis. Their extraction efficiency was taking as index; content was determined by using HPLC chromatography. Finally,L9（3^4）orthogonal design experiment was used to determine the optimum the extraction process. Results：The optimum extraction process：with ethyl acetate as the extraction solvent,0.1 mol/L HCl 3 mL was added into it. Material-liquid ratio was 1∶40（mg/mL）;ultrasonic time 90 min;ultrasonic temperature 40 ℃. Conclusion： The Optimized extraction process is simple,stable and have high extraction rate. |
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| Keywords: | Pinus yunnanensis Faranch pinecone protocatechuic acid orthogonal test |
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