| 日本血吸虫免疫反应中抗原价数及平衡常数Kass的测定 |
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| 引用本文： | 何春萍,吴朝阳,沈国励. 日本血吸虫免疫反应中抗原价数及平衡常数Kass的测定[J]. 怀化学院学报, 1998, 0(5) |
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| 作者姓名： | 何春萍 吴朝阳 沈国励 |
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| 作者单位： | 湖南大学化学计量学与化学传感技术研究所!长沙，410082，湖南大学化学计量学与化学传感技术研究所!长沙，410082 |
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| 摘 要： | 在压电石英晶体金电极表面形成流基丙酸（MPA）单分子膜，经NHS和EDC活化后固定血吸虫抗原（SjAg），研制成血吸虫抗体（SjAb）压电免疫传感器．研究测定了抗原价数及免疫反应平衡常数Kass，结果令人满意．
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| 关 键 词： | 压电免疫传感器 日本血吸虫抗原（SjAg） 日本血吸虫抗体（SjAh）巯基丙酸（MPA） 价数 平衡常数 |
The Detection of the Valent value of SjAg and the affinity Contant of the Immunoreaction |
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| He Chun - ping. The Detection of the Valent value of SjAg and the affinity Contant of the Immunoreaction[J]. Journal of Huaihua University, 1998, 0(5) |
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| Authors: | He Chun - ping |
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| Abstract: | A Piezoelectric immunosensor has ben developed for the determination of SchistlksomaJaponicum antibody (S jAb). The crysha are AT - cut and have basic resoan heency of 9MHz. Immobilization of the SjAg to the crystal's surface was accomglished via a NHA - EDC - activatedcopolymer coating of 3 - Mercaptopropionic acid (MPA). The valent value of SjAg binding with SjAnand the affinity constan of immunoreaction in this experimental system were studied. The analfocal re-sults given by this approarh trere satisfactory- Afer washing tvith Piranha solution, a crystal can be re-used two hundred times without detectable loss of sensitivity |
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| Keywords: | Piezoelectric immunosensor Schistoksoma Japonicum antigen (SjAg ) Schistoksoma Japonicum antibody (SjAb) Metcaptopropionic acid (MPA) valent value affinity constant |
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