裸子植物的生化系统学(一)——松科植物的过氧化物酶

 用聚丙烯酰胺凝胶电泳分析了松科全部10属59种又A变种的过氧化物     酶。  尽管个别种在种内酶谱有变化，但每个种仍有足以与其他种区分的特征性     酶谱。我们定义“两分类群总带数除两分类群不相同带数”为“酶谱距离“，作为     属间和种间酶谱分歧的数量指标。松科各属内平均酶谱距离与Prager等(1976)     报道的抗原距离以及各属的化石历史基本一致。落叶松属、雪松属、金钱松属发     生最晚，云杉属等次之，松属最为古老。分子证据支持属和亚科的划分。对比杨     属的数据，说明过氧化物酶进化有稳定速率。  文章最后讨论了酶谱资料在研究    植物形态进化和分子进化中的作用。

Biochemical Systematics of Gymnosperms (I) Peroxidases of Pinaceae

 By means of polyacrylamide gel electrophoresis, peroxidases of 59 species and 4 varieties, belonging to ten genera of Pinaceae, were analysed. Though intraspecific zymogramatic variation may be observed within a few species, each species possesses its specific zymogram, distinguishable from the others. In order to measure divergence between two taxa, we have defined “zymogram distance” as follows:                                        number of different bands between two taxa   zymogram distance=        ——————————————                                         total number of bands in the same two taxa      Similar tendencies are shown among average intergeneric zymogram distances, antigenic distances (Prager et al. 1976), and generic divergence time. Molecular evidences support classical taxonomy of Pinaceae. Agreed with the data from Populus, the evolutionary rate of plant peroxidase seems stable. The possible contribution of zymogram to  investigation of molecular and morphological evolution of plants is discussed.