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| 荧光波长对双光子荧光共焦成像分辨率的影响 | |
| 引用本文： | 白建平,赵文丽,肖华层. 荧光波长对双光子荧光共焦成像分辨率的影响[J]. 南阳师范学院学报, 2004, 3(6): 24-26 |
| 作者姓名： | 白建平 赵文丽 肖华层 |
| 作者单位： | 1. 南阳师范学院,物理系,河南,南阳,473061 2. 河南省轻工职工大学,河南,郑州,450002 3. 温州师范学院,生物与环境科学系,浙江,温州,325027 |
| 摘 要： | 导出了共焦双光子荧光显微镜探测光强随荧光波长变化的解析表达式。研究了不同荧光波长对双光子荧光共焦显微镜横向、纵向分辨率和光斑强度的影响。结果指出，随着荧光波长与入射波长的比值的增加，横向和纵向分辨率降低，双光子荧光成像的分辨率高于单光子荧光成像的分辨率。 |
| 关 键 词： | 双光子荧光 共焦显微术 分辨率 |
| 文章编号： | 1671-6132(2004)06-0024-03 |
| 修稿时间： | 2004-01-26 |
Effects of Fluorescent Wavelength on the Resolution of Two-photon Fluorescence Confocal Imaging |
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| BAI Jian-ping ZHAO Wen_li XIAO Hua-ceng. Effects of Fluorescent Wavelength on the Resolution of Two-photon Fluorescence Confocal Imaging[J]. Journal of Nanyang Teachers College, 2004, 3(6): 24-26 | |
| Authors: | BAI Jian-ping ZHAO Wen_li XIAO Hua-ceng |
| Affiliation: | BAI Jian-ping~1 ZHAO Wen_li~2 XIAO Hua-ceng~3 |
| Abstract: | The analytic expressions of the fluorescent intensity are derived in two_photon fluorescence confocal imaging. The numerical results show that the transverse resolution, the axial resolution, and the intensity of the spot increase as the rate of fluorescent wavelength to illustrated wavelength increases. The resolution in two_photon fluorescence confocal microscopy is higher than that in single_photon fluorescence confocal microscopy. |
| Keywords: | two_photon fluorescence confocal microscopy resolution |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |