刺五加对甲醛致运动大鼠心肌氧化损伤的保护作用实验研究

目的：通过实验观察甲醛对运动大鼠心肌组织抗氧化系统的影响及服用刺五加后的作用,探讨甲醛对运动大鼠的心肌组织毒性及刺五加的保护作用。方法：采用人为模拟不同程度甲醛染毒环境进行动式染毒,甲醛剂量分别为0.8 mg/m3,2.4 mg/m3。每天染毒30 min,每周6次,连续4周,刺五加的灌胃剂量为104.17 mg/kg,测定运动大鼠心肌组织的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）的活力和丙二醛（MDA）的含量。结果：染毒组与对照组相比,2.4 mg/m3剂量染毒运动组大鼠心肌组织 CAT、GSH-PX、SOD活性明显低于对照组（P〈0.01）,MDA含量明显高于对照组（P〈0.01）。服药染毒运动组与染毒运动组相比,0.8mg/m3剂量染毒服药组大鼠心肌组织CAT、GSH-PX、SOD活性明显高于染毒运动组（P〈0.01）,MDA含量明显低于染毒运动组（P〈0.01）。结论：甲醛可以降低染毒大鼠心肌组织抗氧化酶的活性,造成心肌组织氧化性损伤,而灌服中药刺五加可以有效缓解其毒性。

An Experimental Study on the Protective Effects of Acanthopanax on Formaldehyde Induced Myocardial Oxidative Damage on Rats

Obj ective：Through observing the formaldehyde of Acanthopanax senticosus and af-ter taking the role of anti-oxidation system of rats,to explore neurotoxicity of formaldehyde on the movement of rat and the protective effects of Acanthopanax senticosus.Methods：Using artificial simulation of dynamic contamination of different degrees of formaldehyde,formaldehyde dose was 0. 8mg/m3,2.4mg/m3.Daily exposure to 30min,6 times per week,for 4 weeks,acanthopanax senti-cosus intragastric dose of 104.17mg/kg,superoxide dismutase in heart tissue of rats measured （SOD）,glutathione peroxidase （GSH Px）,catalase （CAT）activity and malondialdehyde （MDA） content.Results：Compared with control group and the control group,the heart tissue in rats of 2. 4mg/m3 group dose CAT,GSH-PX,SOD activity was significantly lower than that of the control group （P〈0.01）,MDA content was significantly higher than that of the control group （P〈0.01）. Compared with the drug contamination movement group and the contamination movement group,0. 8mg/m3 dose drug group rat heart CAT,GSH-PX,SOD activity was significantly higher in the ex-ercise group （P〈0.01）,MDA content was significantly lower than in the exercise group （P〈0.01）. Conclusion：Formaldehyde can decrease the activity of antioxidant enzymes in heart tissue in rats,and cause the oxidative damage of heart tissue,and the perfusion of Chinese medicine Acanthopanax senti-cosus can effectively alleviate the toxicity.