| 分光光度法测定海参中锗的含量 |
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| 引用本文： | 刁全平,高晶,侯冬岩,回瑞华,郭华,佟丽娜.分光光度法测定海参中锗的含量[J].鞍山师范学院学报,2013(4):28-30. |
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| 作者姓名： | 刁全平 高晶 侯冬岩 回瑞华 郭华 佟丽娜 |
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| 作者单位： | [1]鞍山师范学院化学与生命科学学院,辽宁鞍山114007 [2]山东港湾建设集团有限公司,山东日照276826 |
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| 摘 要： | 对不同产地海参中总锗的含量进行测定.采用灰化法对样品进行处理,以CTMAB为增溶剂,明胶为稳定剂,苯芴酮为显色剂,在酸性条件下进行显色,利用分光光度法测定海参中锗的含量.该体系在503 nm处有最大吸收峰,回归方程为A=1.092 5 C+0.168 15,线性范围为0.072~0.504μg/mL,r=0.997 8,方法的回收率为98.54%~99.30%,RSD为1.9%.本方法样品处理简单,准确度高,可用于海参中锗的含量测定.
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| 关 键 词： | 海参 锗 分光光度法 |
Determination of Germanium in Sea Cucumbers by Spectrophotometric Method |
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| DIAO Quan-ping,GAO Jing,HOU Dong-yan,HUI Rui-hua,GUO Hua,TONG Li-na.Determination of Germanium in Sea Cucumbers by Spectrophotometric Method[J].Journal of Anshan Teachers College,2013(4):28-30. |
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| Authors: | DIAO Quan-ping GAO Jing HOU Dong-yan HUI Rui-hua GUO Hua TONG Li-na |
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| Institution: | 1. School of Chemistry and Life Science ,Anshan Normal University,Anshan Liaoning 114007, China; 2. Shandong Harbour Engineering Group Co. ,Lid,Rizhao Shandong 276826, China) |
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| Abstract: | To determine the content of Germanium in Sea Cucumbers, the samples were treated by ashing method, and Germanium was colored with Phenylfluorone in the acidified solution with CTMAB as solubilization agent and gelatine as stabilizer, and the content of Germanium was determined by Spectrophotometry. The maximum absorption of association complex was at 503 nm. Regression equation was A= 1. 092 5C ＋0. 168 15 ,the linear range 0. 072 ～ 0.504 μg/mL, r = 0.997 8, the recovery 98.88% , RSD 1.9%. This method was simple, accurate, and could be used to determine the content of Germanium in Sea Cucumbers. |
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| Keywords: | Sea Cucumbers Germanium Spectrophotometry |
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