| Effect of temperature on batch elastase production by Bacillus sp. EL31410 |
| |
| 作者姓名： | 何国庆 徐莹 陈启和 阮晖 李景军 |
| |
| 作者单位： | Department of Food Science and Nutrition，Zhejiang University，Hangzhou 310029，China，Department of Food Science and Nutrition，Zhejiang University，Hangzhou 310029，China，Department of Food Science and Nutrition，Zhejiang University，Hangzhou 310029，China，Department of Food Science and Nutrition，Zhejiang University，Hangzhou 310029，China，School of Food Science and Technology，Southern Yangtze University，Wuxi 214036，China |
| |
| 基金项目： | Project(No.20276064)supportedbytheNationalNaturalScienceFoundationofChina |
| |
| 摘 要： | The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃ to 28 ℃, were evaluated in shake flask. The result indicated that 37 ℃ was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 ℃. This result was verified by batch fermentation in 5-L bio-reactor under 37 ℃ and 30 ℃ temperature, respectively. The specific cell growth rate at 37 ℃ was higher than that at 30 ℃ during earlier stage of cultivation. The maximum value 5.5 U/(h-g DCW)] of elastase formation rate occurred at 24 h at 30 ℃ compared to 4.6 U/(h-g DCW) at 30 h at 37 ℃. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 ℃ or 30 ℃, both two-stage temperature shift strategy and os
|
Effect of temperature on batch elastase production by Bacillus sp. EL31410 |
| |
| HE Guo-qing,XU Ying CHEN Qi-he RUAN Hui LI Jing-jun.Effect of temperature on batch elastase production by Bacillus sp. EL31410[J].Journal of Zhejiang University Science,2004(12). |
| |
| Authors: | HE Guo-qing XU Ying CHEN Qi-he RUAN Hui LI Jing-jun |
| |
| Abstract: | |
| |
| Keywords: | Bacillus sp EL31410 Elastase Temperature Batch fermentation Temperature-shift strategy |
| 本文献已被 CNKI 等数据库收录！ |
