An integrated microfluidic system for isolation, counting, and sorting of hematopoietic stem cells |
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Authors: | Huei-Wen Wu Ruo-Chi Hsu Chun-Che Lin Shiaw-Min Hwang and Gwo-Bin Lee |
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Institution: | 1Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan;2Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan |
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Abstract: | This study reports an integrated microfluidic system capable of isolation, counting, and sorting of hematopoietic stem cells (HSCs) from cord blood in an automatic format by utilizing a magnetic-bead-based immunoassay. Three functional modules, including cell isolation, cell counting, and cell sorting modules are integrated on a single chip by using microfluidic technology. The cell isolation module is comprised of a four-membrane-type micromixer for binding of target stem cells and magnetic beads, two pneumatic micropumps for sample transport, and an S-shaped channel for isolation of HSCs using a permanent magnet underneath. The counting and sorting of HSCs are performed by utilizing the cell counting and sorting modules. Experimental results show that a separation efficiency as high as 88% for HSCs from cord blood is achieved within 40 min for a sample volume of 100 μl. Therefore, the development of this integrated microfluidic system may be promising for various applications such as stem cell research and cell therapy. |
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