The role of DNA diffusion in solid phase polymerase chain reaction with gel-immobilized primers in planar and capillary microarray format |
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Authors: | Alexei L Drobyshev Tatiana V Nasedkina and Natalia V Zakharova |
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Institution: | 1Engelhardt Institute of Molecular Biology, Vavilov Str. 32, 119991 Moscow, Russia;2State Research Institute of Genetics, 1st Dorozhny Pr. 1, 117545 Moscow, Russia |
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Abstract: | The solid phase polymerase chain reaction (PCR) on a gel-based microarray system was studied under various durations of individual stages of the PCR cycle and spatial restriction of the reaction volume. Combining the experimental study with numerical modeling, we demonstrated that the diffusion of the PCR product in and out of a microarray element during the annealing and melting stages, respectively, is the main factor responsible for distinctive features of the studied type of PCR. The restriction of reaction volume leads to faster PCR signal growth. Particularly, the capillary array, whereby gel-based microarray elements are located on a glass bar inserted into capillary chamber, was found to be a suitable format for the development of the platform. |
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