Molecular cloning and nucleotides sequence analysis of G1 genome segment of hantaan virus Z10 strain |
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Authors: | Liang Wei-feng Shen Yue-hong Ma Yi-lin Zhao Nian-feng Chen Ya-gang Zhu Zhi-yong Xu Xiao |
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Institution: | (1) The First Affiliated Hospital of Zhejiang University, 310003 Hangzhou, China;(2) Zhejiang Health and Anti-epidemic Station, 310006 Hangzhou, China;(3) University of California, USA |
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Abstract: | The molecular cloning of the G1 genome segment of the Z10 strain of Hantaan virus and analysis of the first gene data on the currently widely used Hantavirus vaccine strain in China
are presented. The G1 genome segment of Z10 virus was amplified by the method of RT-PCR, and the products were cloned into the pGEM-T vector after identification and
purification. The clone was sequenced by Sanger’s dideoxy chain termination method. The 1449 bases of the Z10 G1 genome segment, and the coding for 483 amino acids were determined. The base compositions for the G1 segment RNA determined from cDNA sequence information, were 20.6% A, 21.6% G, 18.8% C and 30.0% U. These values are similar
to those of the 76/118, Lee and SR virus. As compared to the Z10 G1 segment, the sequence homology at the nucleotide level is 87% (76/118, type I), 86% (Lee, type I), 86% (Hojo, type I), 67%
(R22, type II), and 59% (K22, type III). The Z10 virus G1 protein amino acid sequence identity with other Hantaan viruses (94–95% homology) was higher than that with Seoul type viruses
(77–80%). More amino acid sequence heterogeneity between the Z10 and 76/118 was observed in the N terminal, especially the diverging cluster of ten amino acids at position 84 to 93 of the
Z10 virus G1 protein. Conclusion: (1) The Z10 strain is one of the Hantaan viruses. (2) The important region of the Z10 G1 segment was conservative. (3) Although substantial divergence of nucleotide sequences of the G1 genome segment was found between the Z10 strain and other type I Hantaviruses, relatively high amino acid sequence homology was shown among them. Thus, good immune
protection could be obtained with the inactivated Meriones unguiculatus kidney cell vaccine against other strains of Hantaviruses. |
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