Expression of a bee venom phospholipase A2 from Apis cerana cerana in the baculovirus-insect cell |
| |
| Authors: | Li-rong Shen Mei-hui Ding Li-wen Zhang Wei-guang Zhang Liang Liu Duo Li |
| |
| Affiliation: | (1) Department of Zoology and Entomology, Rhodes University, Grahamstown, 6140, South Africa |
| |
| Abstract: | Bee venom phospholipase A2(BvPLA2) is a lipolytic enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerophospholipids to liberate free fatty acids and lysophospholipids.In this work,a new BvPLA2(AccPLA2) gene from the Chinese honeybee(Apis cerana cerana) venom glands was inserted into bacmid to construct a recombinant transfer vector.Tn-5B-4(Tn) cells were transfected with the recombinant bacmid DNA for expression.Sodium dodecylsulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis rev... |
| |
| Keywords: | Apis cerana cerana Bee venom phospholipase A2(BvPLA2) Insect cell Expression |
| 本文献已被 CNKI 万方数据 SpringerLink 等数据库收录！ |
