Expression and preliminary functional analysis of Siglec-F on mouse macrophages |
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Authors: | Yin-he Feng and Hui Mao |
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Institution: | Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China |
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Abstract: | Sialic acid-binding immunoglobulin-like lectin (Siglec)-F is a mouse functional paralog of human Siglec-8 that induces apoptosis
in human eosinophils, and therefore may be useful as the basis of treatments for a variety of disorders associated with eosinophil
hyperactivity, such as asthma. The expression pattern and functions of this protein in various cell types remain to be elucidated.
The aim of this study was to determine the expression of Siglec-F on mouse macrophages by immunocytochemical staining, and
also to investigate the effects of Siglec-F engagement by a Siglec-F antibody on phagocytic activity of macrophages. The results
showed that Siglec-F expression was detected on mouse alveolar macrophages, but not on peritoneal macrophages. Furthermore,
Siglec-F engagement did not affect the phagocytic activity of alveolar macrophages in the resting state or in the activated
state following stimulation by the proinflammatory mediator tumor necrosis factor alpha (TNF-α) or lipopolysaccharide (LPS).
Siglec-F expression on alveolar macrophages may be a result of adaptation. Macrophages actively regulate immune responses
via production of cytokines. Therefore, further investigation of the effects of Siglec-F engagement on immune mediators or
cytokines released by alveolar macrophages is required. |
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Keywords: | Siglec-F Expression Macrophages Phagocytosis Eosinophil |
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