蜘蛛染色体制片技术探索

本文以多种蜘蛛不同的组织器官为实验材料，通过设置不同低渗处理时间和采用多种固定的方法，旨在找到蜘蛛染色体制片技术的最优化方案．结果表明：选取材料以蜘蛛处于发育中的生殖腺和幼蛛的附肢细胞为最好．在无水浴条件下，低渗时间对取血细胞为40分钟，对幼蛛取45分钟为最佳．用新鲜固定液进行固定，对第一次固定加以改进，采用高比例甲醇固定剂处理，有利于染色体伸展和染色体分散，得到理想的分裂相．

SMEARING TECHNOLOGY FOR SPIDER CHROMOSOME

In order to find the best smearing technology for spider chromosome,experiments have made by using different tissues and organs of diverse spiders as experimental materials,and designing different low-osmosis work-time and diverse fixed methods. Results indicate that the growing spider's reproduction gonad and young spider's appendage cell are thebest experimental materials; without water-bath, the low-osmosis work-time is 40 minutesfor blood cell (but 45 minutes for young spiders),and when high concentrated methanol isused as the first fixing-solution, it is of advantage for extension and dispersion of chromosome,and it is easy to gain the satisfactory divided-phase.